Overview
| Uniprot ID | P39656 |
|---|
| Protein Name | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit |
|---|
| Gene Name | DDOST |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 189 |
KAPTIVGKSSLNPIL |
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes (PubMed:22467853)
Protein Sequence
10
MGYFRCARAG
20
SFGRRRKMEP
30
STAARAWALF
40
WLLLPLLGAV
50
CASGPRTLVL
60
LDNLNVRETH
70
SLFFRSLKDR
80
GFELTFKTAD
90
DPSLSLIKYG
100
EFLYDNLIIF
110
SPSVEDFGGN
120
INVETISAFI
130
DGGGSVLVAA
140
SSDIGDPLRE
150
LGSECGIEFD
160
EEKTAVIDHH
170
NYDISDLGQH
180
TLIVADTENL
190
LKAPTIVGKS
200
SLNPILFRGV
210
GMVADPDNPL
220
VLDILTGSST
230
SYSFFPDKPI
240
TQYPHAVGKN
250
TLLIAGLQAR
260
NNARVIFSGS
270
LDFFSDSFFN
280
SAVQKAAPGS
290
QRYSQTGNYE
300
LAVALSRWVF
310
KEEGVLRVGP
320
VSHHRVGETA
330
PPNAYTVTDL
340
VEYSIVIQQL
350
SNGKWVPFDG
360
DDIQLEFVRI
370
DPFVRTFLKK
380
KGGKYSVQFK
390
LPDVYGVFQF
400
KVDYNRLGYT
410
HLYSSTQVSV
420
RPLQHTQYER
430
FIPSAYPYYA
440
SAFSMMLGLF
450
IFSIVFLHMK
EKEKSD
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035577 |
azurophil granule membrane |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0043231 |
intracellular membrane-bounded organelle |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0008250 |
oligosaccharyltransferase complex |
| Cellular Component |
GO:0160226 |
oligosaccharyltransferase complex A |
| Cellular Component |
GO:0160227 |
oligosaccharyltransferase complex B |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0008047 |
enzyme activator activity |
| Biological Process |
GO:0009101 |
glycoprotein biosynthetic process |
| Biological Process |
GO:0006487 |
protein N-linked glycosylation |
| Biological Process |
GO:0031647 |
regulation of protein stability |
| Biological Process |
GO:0034097 |
response to cytokine |
| Biological Process |
GO:0042110 |
T cell activation |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.