Overview
| Uniprot ID | P40630 |
|---|
| Protein Name | Transcription factor A, mitochondrial |
|---|
| Gene Name | Tfam |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 82 |
KLSELVRKIAALWRE |
| 96 |
ELPEAEKKVYEADFK |
Function
Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (By similarity). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (By similarity). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (By similarity). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (By similarity). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (By similarity). Is able to unwind DNA (By similarity). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (By similarity). Required for maintenance of normal levels of mitochondrial DNA (PubMed:9500544). May play a role in organizing and compacting mitochondrial DNA (PubMed:17581862)
Protein Sequence
10
MALFRGMWSV
20
LKALGRTGVE
30
MCAGCGGRIP
40
SSISLVCIPK
50
CFSSMGSYPK
60
KPMSSYLRFS
70
TEQLPKFKAK
80
HPDAKLSELV
90
RKIAALWREL
100
PEAEKKVYEA
110
DFKAEWKAYK
120
EAVSKYKEQL
130
TPSQLMGMEK
140
EARQRRLKKK
150
ALVKRRELIL
160
LGKPKRPRSA
170
YNIYVSESFQ
180
EAKDDSAQGK
190
LKLVNEAWKN
200
LSPEEKQAYI
210
QLAKDDRIRY
220
DNEMKSWEEQ
230
MAEVGRSDLI
240
RRSVKRSGDI
SEH
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0042645 |
mitochondrial nucleoid |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0031072 |
heat shock protein binding |
| Molecular Function |
GO:0001018 |
mitochondrial promoter sequence-specific DNA binding |
| Molecular Function |
GO:0034246 |
mitochondrial transcription factor activity |
| Molecular Function |
GO:0043565 |
sequence-specific DNA binding |
| Molecular Function |
GO:0001223 |
transcription coactivator binding |
| Biological Process |
GO:0033108 |
mitochondrial respiratory chain complex assembly |
| Biological Process |
GO:0006390 |
mitochondrial transcription |
| Biological Process |
GO:0001666 |
response to hypoxia |
| Biological Process |
GO:0007584 |
response to nutrient |
| Biological Process |
GO:0006391 |
transcription initiation at mitochondrial promoter |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.