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Overview

Uniprot IDP41252
Protein NameIsoleucine--tRNA ligase, cytoplasmic
Gene NameIARS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
606 QKMSKRKKNYPDPVS

Function

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA

Protein Sequence

10 MLQQVPENIN 20 FPAEEEKILE 30 FWTEFNCFQE 40 CLKQSKHKPK 50 FTFYDGPPFA 60 TGLPHYGHIL 70 AGTIKDIVTR 80 YAHQSGFHVD 90 RRFGWDCHGL 100 PVEYEIDKTL 110 GIRGPEDVAK 120 MGITEYNNQC 130 RAIVMRYSAE 140 WKSTVSRLGR 150 WIDFDNDYKT 160 LYPQFMESVW 170 WVFKQLYDKG 180 LVYRGVKVMP 190 FSTACNTPLS 200 NFESHQNYKD 210 VQDPSVFVTF 220 PLEEDETVSL 230 VAWTTTPWTL 240 PSNLAVCVNP 250 EMQYVKIKDV 260 ARGRLLILME 270 ARLSALYKLE 280 SDYEILERFP 290 GAYLKGKKYR 300 PLFDYFLKCK 310 ENGAFTVLVD 320 NYVKEEEGTG 330 VVHQAPYFGA 340 EDYRVCMDFN 350 IIRKDSLPVC 360 PVDASGCFTT 370 EVTDFAGQYV 380 KDADKSIIRT 390 LKEQGRLLVA 400 TTFTHSYPFC 410 WRSDTPLIYK 420 AVPSWFVRVE 430 NMVDQLLRNN 440 DLCYWVPELV 450 REKRFGNWLK 460 DARDWTISRN 470 RYWGTPIPLW 480 VSDDFEEVVC 490 IGSVAELEEL 500 SGAKISDLHR 510 ESVDHLTIPS 520 RCGKGSLHRI 530 SEVFDCWFES 540 GSMPYAQVHY 550 PFENKREFED 560 AFPADFIAEG 570 IDQTRGWFYT 580 LLVLATALFG 590 QPPFKNVIVN 600 GLVLASDGQK 610 MSKRKKNYPD 620 PVSIIQKYGA 630 DALRLYLINS 640 PVVRAENLRF 650 KEEGVRDVLK 660 DVLLPWYNAY 670 RFLIQNVLRL 680 QKEEEIEFLY 690 NENTVRESPN 700 ITDRWILSFM 710 QSLIGFFETE 720 MAAYRLYTVV 730 PRLVKFVDIL 740 TNWYVRMNRR 750 RLKGENGMED 760 CVMALETLFS 770 VLLSLCRLMA 780 PYTPFLTELM 790 YQNLKVLIDP 800 VSVQDKDTLS 810 IHYLMLPRVR 820 EELIDKKTES 830 AVSQMQSVIE 840 LGRVIRDRKT 850 IPIKYPLKEI 860 VVIHQDPEAL 870 KDIKSLEKYI 880 IEELNVRKVT 890 LSTDKNKYGI 900 RLRAEPDHMV 910 LGKRLKGAFK 920 AVMTSIKQLS 930 SEELEQFQKT 940 GTIVVEGHEL 950 HDEDIRLMYT 960 FDQATGGTAQ 970 FEAHSDAQAL 980 VLLDVTPDQS 990 MVDEGMAREV 1000 INRIQKLRKK 1010 CNLVPTDEIT 1020 VYYKAKSEGT 1030 YLNSVIESHT 1040 EFIFTTIKAP 1050 LKPYPVSPSD 1060 KVLIQEKTQL 1070 KGSELEITLT 1080 RGSSLPGPAC 1090 AYVNLNICAN 1100 GSEQGGVLLL 1110 ENPKGDNRLD 1120 LLKLKSVVTS 1130 IFGVKNTELA 1140 VFHDETEIQN 1150 QTDLLSLSGK 1160 TLCVTAGSAP 1170 SLINSSSTLL 1180 CQYINLQLLN 1190 AKPQECLMGT 1200 VGTLLLENPL 1210 GQNGLTHQGL 1220 LYEAAKVFGL 1230 RSRKLKLFLN 1240 ETQTQEITED 1250 IPVKTLNMKT 1260 VYVSVLPTTA DF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0016020 membrane
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0051020 GTPase binding
Molecular Function GO:0004822 isoleucine-tRNA ligase activity
Molecular Function GO:0000049 tRNA binding
Biological Process GO:0006428 isoleucyl-tRNA aminoacylation
Biological Process GO:0001649 osteoblast differentiation
Biological Process GO:0006418 tRNA aminoacylation for protein translation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.