Search Results

Overview

Uniprot IDP42125
Protein NameEnoyl-CoA delta isomerase 1, mitochondrial
Gene NameEci1
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
222 PEDQVHSKARSVMTK
229 KARSVMTKWLAIPDH
247 LTKNMMRKATADNLI
255 ATADNLIKQREADIQ
275 ISKDSIQKSLHMYLE
283 SLHMYLEKLKQKKG*
76 LEKLENDKSIRGVIL

Function

Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids. Together with the 2,4-dienoyl-CoA reductase (DECR) and the Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (ECH1), they allow the reentrance of the enoyl-CoA into the beta-oxidation cycle. Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of fatty enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (By similarity). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length, and based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (By similarity)

Protein Sequence

10 MALAAARRLL 20 LHAGSRLGRR 30 EAVDGARRFA 40 NKRVLVETEG 50 PAGVAVMKLR 60 NPPVNSLSLE 70 CLTEFTISLE 80 KLENDKSIRG 90 VILTSECPGI 100 FSAGLDLLEM 110 YGRNPAHYAE 120 YWKNVQELWL 130 RLYTSNMILV 140 SAINGASPAG 150 GCLLALCCDY 160 RVMADNPKYT 170 IGLNESLLGI 180 VAPFWFKDMY 190 VNTIGHREAE 200 RALQLGTLFS 210 PAEALKVGVV 220 DEVVPEDQVH 230 SKARSVMTKW 240 LAIPDHSRQL 250 TKNMMRKATA 260 DNLIKQREAD 270 IQNFTSFISK 280 DSIQKSLHMY LEKLKQKKG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0016863 intramolecular oxidoreductase activity, transposing C=C bonds
Biological Process GO:0006635 fatty acid beta-oxidation

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.