Search Results

Overview

Uniprot ID	P42126
Protein Name	Enoyl-CoA delta isomerase 1, mitochondrial
Gene Name	ECI1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
288	ISKDSIQKSLQMYLE
61	GAGVAVMKFKNPPVN
89	LEKLENDKSFRGVIL

Function

Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids (PubMed:26474213, PubMed:7818490). Together with the 2,4-dienoyl-CoA reductase (DECR) and the Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase (ECH1), they allow the reentrance of the enoyl-CoA into the beta-oxidation cycle (PubMed:26474213). Able to isomerize both 3-cis (3Z) and 3-trans (3E) double bonds into the 2-trans (2E) form in a range of fatty enoyl-CoA species, with a preference for (3Z)-enoyl-CoAs over (3E)-enoyl-CoAs (PubMed:26474213, PubMed:7818490). The catalytic efficiency of this enzyme is not affected by the fatty acyl chain length, and based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (By similarity)

Protein Sequence

10 MALVASVRVP 20 ARVLLRAGAR 30 LPGAALGRTE 40 RAAGGGDGAR 50 RFGSQRVLVE 60 PDAGAGVAVM 70 KFKNPPVNSL 80 SLEFLTELVI 90 SLEKLENDKS 100 FRGVILTSDR 110 PGVFSAGLDL 120 TEMCGRSPAH 130 YAGYWKAVQE 140 LWLRLYQSNL 150 VLVSAINGAC 160 PAGGCLVALT 170 CDYRILADNP 180 RYCIGLNETQ 190 LGIIAPFWLK 200 DTLENTIGHR 210 AAERALQLGL 220 LFPPAEALQV 230 GIVDQVVPEE 240 QVQSTALSAI 250 AQWMAIPDHA 260 RQLTKAMMRK 270 ATASRLVTQR 280 DADVQNFVSF 290 ISKDSIQKSL 300 QMYLERLKEE KG

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0004165	delta(3)-delta(2)-enoyl-CoA isomerase activity
Molecular Function	GO:0016863	intramolecular oxidoreductase activity, transposing C=C bonds
Biological Process	GO:0006635	fatty acid beta-oxidation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.