Search Results

Overview

Uniprot IDP43155
Protein NameCarnitine O-acetyltransferase
Gene NameCRAT
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
186 SCRVPGPKQDTVSNF
195 DTVSNFSKTKKPPTH
245 NSSLQTNKEPVGILT
261 NHRNSWAKAYNTLIK
268 KAYNTLIKDKVNRDS
390 FNITPEIKSDIEKAK
609 RLAHYLEKALLDMRA
85 GVGERLQKGLERRAR
93 GLERRARKTENWLSE

Function

Catalyzes the reversible transfer of acyl groups from carnitine to coenzyme A (CoA) and regulates the acyl-CoA/CoA ratio. Also plays a crucial role in the transport of fatty acids for beta-oxidation (PubMed:15099582, PubMed:29395073). Responsible for the synthesis of short- and branched-chain acylcarnitines (PubMed:23485643). Active towards some branched-chain amino acid oxidation pathway (BCAAO) intermediates (PubMed:23485643). Trans-2-enoyl-CoAs and 2-methylacyl-CoAs are poor substrates (PubMed:23485643)

Protein Sequence

10 MLAFAARTVV 20 KPLGFLKPFS 30 LMKASSRFKA 40 HQDALPRLPV 50 PPLQQSLDHY 60 LKALQPIVSE 70 EEWAHTKQLV 80 DEFQASGGVG 90 ERLQKGLERR 100 ARKTENWLSE 110 WWLKTAYLQY 120 RQPVVIYSSP 130 GVMLPKQDFV 140 DLQGQLRFAA 150 KLIEGVLDFK 160 VMIDNETLPV 170 EYLGGKPLCM 180 NQYYQILSSC 190 RVPGPKQDTV 200 SNFSKTKKPP 210 THITVVHNYQ 220 FFELDVYHSD 230 GTPLTADQIF 240 VQLEKIWNSS 250 LQTNKEPVGI 260 LTSNHRNSWA 270 KAYNTLIKDK 280 VNRDSVRSIQ 290 KSIFTVCLDA 300 TMPRVSEDVY 310 RSHVAGQMLH 320 GGGSRLNSGN 330 RWFDKTLQFI 340 VAEDGSCGLV 350 YEHAAAEGPP 360 IVTLLDYVIE 370 YTKKPELVRS 380 PLVPLPMPKK 390 LRFNITPEIK 400 SDIEKAKQNL 410 SIMIQDLDIT 420 VMVFHHFGKD 430 FPKSEKLSPD 440 AFIQMALQLA 450 YYRIYGQACA 460 TYESASLRMF 470 HLGRTDTIRS 480 ASMDSLTFVK 490 AMDDSSVTEH 500 QKVELLRKAV 510 QAHRGYTDRA 520 IRGEAFDRHL 530 LGLKLQAIED 540 LVSMPDIFMD 550 TSYAIAMHFH 560 LSTSQVPAKT 570 DCVMFFGPVV 580 PDGYGVCYNP 590 MEAHINFSLS 600 AYNSCAETNA 610 ARLAHYLEKA 620 LLDMRALLQS HPRAKL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005782 peroxisomal matrix
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0003997 acyl-CoA oxidase activity
Molecular Function GO:0004092 carnitine O-acetyltransferase activity
Molecular Function GO:0008458 carnitine O-octanoyltransferase activity
Biological Process GO:0019254 carnitine metabolic process, CoA-linked
Biological Process GO:0033540 fatty acid beta-oxidation using acyl-CoA oxidase
Biological Process GO:0051791 medium-chain fatty acid metabolic process
Biological Process GO:0046459 short-chain fatty acid metabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.