Search Results

Overview

Uniprot IDP43490
Protein NameNicotinamide phosphoribosyltransferase
Gene NameNAMPT
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
228 VAGLALIKKYYGTKD
229 AGLALIKKYYGTKDP
331 VLEILGKKFPVTENS
339 FPVTENSKGYKLLPP
423 DPVADPNKRSKKGRL
469 FKNGKVTKSYSFDEI
478 YSFDEIRKNAQLNIE
48 EKKTENSKLRKVKYE

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity)

Protein Sequence

10 MNPAAEAEFN 20 ILLATDSYKV 30 THYKQYPPNT 40 SKVYSYFECR 50 EKKTENSKLR 60 KVKYEETVFY 70 GLQYILNKYL 80 KGKVVTKEKI 90 QEAKDVYKEH 100 FQDDVFNEKG 110 WNYILEKYDG 120 HLPIEIKAVP 130 EGFVIPRGNV 140 LFTVENTDPE 150 CYWLTNWIET 160 ILVQSWYPIT 170 VATNSREQKK 180 ILAKYLLETS 190 GNLDGLEYKL 200 HDFGYRGVSS 210 QETAGIGASA 220 HLVNFKGTDT 230 VAGLALIKKY 240 YGTKDPVPGY 250 SVPAAEHSTI 260 TAWGKDHEKD 270 AFEHIVTQFS 280 SVPVSVVSDS 290 YDIYNACEKI 300 WGEDLRHLIV 310 SRSTQAPLII 320 RPDSGNPLDT 330 VLKVLEILGK 340 KFPVTENSKG 350 YKLLPPYLRV 360 IQGDGVDINT 370 LQEIVEGMKQ 380 KMWSIENIAF 390 GSGGGLLQKL 400 TRDLLNCSFK 410 CSYVVTNGLG 420 INVFKDPVAD 430 PNKRSKKGRL 440 SLHRTPAGNF 450 VTLEEGKGDL 460 EEYGQDLLHT 470 VFKNGKVTKS 480 YSFDEIRKNA 490 QLNIELEAAH H

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005125 cytokine activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0047280 nicotinamide phosphoribosyltransferase activity
Biological Process GO:0007267 cell-cell signaling
Biological Process GO:0032922 circadian regulation of gene expression
Biological Process GO:0006954 inflammatory response
Biological Process GO:0009435 NAD+ biosynthetic process
Biological Process GO:0034355 NAD+ biosynthetic process via the salvage pathway
Biological Process GO:0006741 NADP+ biosynthetic process
Biological Process GO:1901847 nicotinate metabolic process
Biological Process GO:0043123 positive regulation of canonical NF-kappaB signal transduction
Biological Process GO:0008284 positive regulation of cell population proliferation
Biological Process GO:0070374 positive regulation of ERK1 and ERK2 cascade
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0007165 signal transduction

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[5] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.