Search Results

Overview

Uniprot IDP45954
Protein NameShort/branched chain specific acyl-CoA dehydrogenase, mitochondrial
Gene NameACADSB
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
152 LINTLIRKHGTEEQK
159 KHGTEEQKATYLPQL
194 ALKTRADKEGDYYVL
248 TPGLHIGKPENKLGL
252 HIGKPENKLGLRASS
284 GQIGHGYKYAIGSLN
371 IKEASMAKYYASEIA
70 MIKSSVKKFAQEQIA
88 STMDENSKMEKSVIQ

Function

Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the proR-proR stereospecific alpha,beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA) (PubMed:10832746, PubMed:11013134, PubMed:21430231, PubMed:7698750). Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (butanoyl-CoA) (PubMed:10832746, PubMed:11013134, PubMed:21430231, PubMed:7698750). Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2-methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway (PubMed:10832746, PubMed:11013134). Can also act on valproyl-CoA, a metabolite of valproic acid, an antiepileptic drug (PubMed:8660691)

Protein Sequence

10 MEGLAVRLLR 20 GSRLLRRNFL 30 TCLSSWKIPP 40 HVSKSSQSEA 50 LLNITNNGIH 60 FAPLQTFTDE 70 EMMIKSSVKK 80 FAQEQIAPLV 90 STMDENSKME 100 KSVIQGLFQQ 110 GLMGIEVDPE 120 YGGTGASFLS 130 TVLVIEELAK 140 VDASVAVFCE 150 IQNTLINTLI 160 RKHGTEEQKA 170 TYLPQLTTEK 180 VGSFCLSEAG 190 AGSDSFALKT 200 RADKEGDYYV 210 LNGSKMWISS 220 AEHAGLFLVM 230 ANVDPTIGYK 240 GITSFLVDRD 250 TPGLHIGKPE 260 NKLGLRASST 270 CPLTFENVKV 280 PEANILGQIG 290 HGYKYAIGSL 300 NEGRIGIAAQ 310 MLGLAQGCFD 320 YTIPYIKERI 330 QFGKRLFDFQ 340 GLQHQVAHVA 350 TQLEAARLLT 360 YNAARLLEAG 370 KPFIKEASMA 380 KYYASEIAGQ 390 TTSKCIEWMG 400 GVGYTKDYPV 410 EKYFRDAKIG 420 TIYEGASNIQ 430 LNTIAKHIDA EY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0003995 acyl-CoA dehydrogenase activity
Molecular Function GO:0050660 flavin adenine dinucleotide binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003853 short-chain 2-methyl fatty acyl-CoA dehydrogenase activity
Molecular Function GO:0016937 short-chain fatty acyl-CoA dehydrogenase activity
Biological Process GO:0006631 fatty acid metabolic process
Biological Process GO:0006550 L-isoleucine catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.