Search Results

Overview

Uniprot IDP46063
Protein NameATP-dependent DNA helicase Q1
Gene NameRECQL
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
193 LIYVTPEKIAKSKMF
198 PEKIAKSKMFMSRLE
618 GDKKMEEKNSGNFQK
625 KNSGNFQKKAANMLQ
626 NSGNFQKKAANMLQQ
637 MLQQSGSKNTGAKKR

Function

DNA helicase that plays a role in DNA damage repair and genome stability (PubMed:15886194, PubMed:35025765, PubMed:7527136, PubMed:7961977, PubMed:8056767). Exhibits a Mg(2+)- and ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:19151156, PubMed:35025765, PubMed:7527136, PubMed:8056767). Full-length protein unwinds forked DNA substrates, resolves Holliday junctions, and has DNA strand annealing activity (PubMed:19151156, PubMed:25831490). Plays a role in restoring regressed replication forks (PubMed:35025765). Required to restart stalled replication forks induced by abortive topoisomerase 1 and 2 lesions (PubMed:35025765). Does not unwind G-quadruplex DNA (PubMed:18426915). May play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens (PubMed:15886194, PubMed:7961977)

Protein Sequence

10 MASVSALTEE 20 LDSITSELHA 30 VEIQIQELTE 40 RQQELIQKKK 50 VLTKKIKQCL 60 EDSDAGASNE 70 YDSSPAAWNK 80 EDFPWSGKVK 90 DILQNVFKLE 100 KFRPLQLETI 110 NVTMAGKEVF 120 LVMPTGGGKS 130 LCYQLPALCS 140 DGFTLVICPL 150 ISLMEDQLMV 160 LKQLGISATM 170 LNASSSKEHV 180 KWVHAEMVNK 190 NSELKLIYVT 200 PEKIAKSKMF 210 MSRLEKAYEA 220 RRFTRIAVDE 230 VHCCSQWGHD 240 FRPDYKALGI 250 LKRQFPNASL 260 IGLTATATNH 270 VLTDAQKILC 280 IEKCFTFTAS 290 FNRPNLYYEV 300 RQKPSNTEDF 310 IEDIVKLING 320 RYKGQSGIIY 330 CFSQKDSEQV 340 TVSLQNLGIH 350 AGAYHANLEP 360 EDKTTVHRKW 370 SANEIQVVVA 380 TVAFGMGIDK 390 PDVRFVIHHS 400 MSKSMENYYQ 410 ESGRAGRDDM 420 KADCILYYGF 430 GDIFRISSMV 440 VMENVGQQKL 450 YEMVSYCQNI 460 SKCRRVLMAQ 470 HFDEVWNSEA 480 CNKMCDNCCK 490 DSAFERKNIT 500 EYCRDLIKIL 510 KQAEELNEKL 520 TPLKLIDSWM 530 GKGAAKLRVA 540 GVVAPTLPRE 550 DLEKIIAHFL 560 IQQYLKEDYS 570 FTAYATISYL 580 KIGPKANLLN 590 NEAHAITMQV 600 TKSTQNSFRA 610 ESSQTCHSEQ 620 GDKKMEEKNS 630 GNFQKKAANM 640 LQQSGSKNTG AKKRKIDDA

Gene Ontology

Classification GO ID Description
Molecular Function GO:1990814 DNA/DNA annealing activity
Cellular Component GO:0005694 chromosome
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0016020 membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0043138 3'-5' DNA helicase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:0036121 double-stranded DNA helicase activity
Molecular Function GO:0009378 four-way junction helicase activity
Molecular Function GO:0046872 metal ion binding
Biological Process GO:0006281 DNA repair
Biological Process GO:0006260 DNA replication
Biological Process GO:0000724 double-strand break repair via homologous recombination
Biological Process GO:0031297 replication fork processing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[6] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[7] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[8] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[9] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.