Search Results

Overview

Uniprot IDP46459
Protein NameVesicle-fusing ATPase
Gene NameNSF
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
105 EIDFLQKKSIDSNPY
161 AMDPSILKGEPATGK
198 SSLNLIGKAKTKENR
293 NGPEILNKYVGESEA
304 ESEANIRKLFADAEE
427 LSADVDIKELAVETK
549 EGPPHSGKTALAAKI
699 TTIAQQVKGKKVWIG
701 IAQQVKGKKVWIGIK

Function

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity)

Protein Sequence

10 MAGRSMQAAR 20 CPTDELSLTN 30 CAVVNEKDFQ 40 SGQHVIVRTS 50 PNHRYTFTLK 60 THPSVVPGSI 70 AFSLPQRKWA 80 GLSIGQEIEV 90 SLYTFDKAKQ 100 CIGTMTIEID 110 FLQKKSIDSN 120 PYDTDKMAAE 130 FIQQFNNQAF 140 SVGQQLVFSF 150 NEKLFGLLVK 160 DIEAMDPSIL 170 KGEPATGKRQ 180 KIEVGLVVGN 190 SQVAFEKAEN 200 SSLNLIGKAK 210 TKENRQSIIN 220 PDWNFEKMGI 230 GGLDKEFSDI 240 FRRAFASRVF 250 PPEIVEQMGC 260 KHVKGILLYG 270 PPGCGKTLLA 280 RQIGKMLNAR 290 EPKVVNGPEI 300 LNKYVGESEA 310 NIRKLFADAE 320 EEQRRLGANS 330 GLHIIIFDEI 340 DAICKQRGSM 350 AGSTGVHDTV 360 VNQLLSKIDG 370 VEQLNNILVI 380 GMTNRPDLID 390 EALLRPGRLE 400 VKMEIGLPDE 410 KGRLQILHIH 420 TARMRGHQLL 430 SADVDIKELA 440 VETKNFSGAE 450 LEGLVRAAQS 460 TAMNRHIKAS 470 TKVEVDMEKA 480 ESLQVTRGDF 490 LASLENDIKP 500 AFGTNQEDYA 510 SYIMNGIIKW 520 GDPVTRVLDD 530 GELLVQQTKN 540 SDRTPLVSVL 550 LEGPPHSGKT 560 ALAAKIAEES 570 NFPFIKICSP 580 DKMIGFSETA 590 KCQAMKKIFD 600 DAYKSQLSCV 610 VVDDIERLLD 620 YVPIGPRFSN 630 LVLQALLVLL 640 KKAPPQGRKL 650 LIIGTTSRKD 660 VLQEMEMLNA 670 FSTTIHVPNI 680 ATGEQLLEAL 690 ELLGNFKDKE 700 RTTIAQQVKG 710 KKVWIGIKKL 720 LMLIEMSLQM 730 DPEYRVRKFL 740 ALLREEGASP LDFD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0043198 dendritic shaft
Cellular Component GO:0098982 GABA-ergic synapse
Cellular Component GO:0005795 Golgi stack
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0099091 postsynaptic specialization, intracellular component
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0140545 ATP-dependent protein disaggregase activity
Molecular Function GO:0035255 ionotropic glutamate receptor binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0030165 PDZ domain binding
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0000149 SNARE binding
Molecular Function GO:0017075 syntaxin-1 binding
Biological Process GO:0006887 exocytosis
Biological Process GO:0043001 Golgi to plasma membrane protein transport
Biological Process GO:0006891 intra-Golgi vesicle-mediated transport
Biological Process GO:0006886 intracellular protein transport
Biological Process GO:0045026 plasma membrane fusion
Biological Process GO:0045732 positive regulation of protein catabolic process
Biological Process GO:0001921 positive regulation of receptor recycling
Biological Process GO:0017157 regulation of exocytosis
Biological Process GO:0035494 SNARE complex disassembly
Biological Process GO:0016192 vesicle-mediated transport

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[3] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[4] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[7] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.