Overview
| Uniprot ID | P47962 |
|---|
| Protein Name | Large ribosomal subunit protein uL18 |
|---|
| Gene Name | Rpl5 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 276 |
PKMSLAQKKDRVAQK |
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:36517592). The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules (By similarity). The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain (By similarity). The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel (By similarity). As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs (By similarity). It also couples ribosome biogenesis to p53/TP53 activation (By similarity). As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53 (By similarity)
Protein Sequence
10
MGFVKVVKNK
20
AYFKRYQVRF
30
RRRREGKTDY
40
YARKRLVIQD
50
KNKYNTPKYR
60
MIVRVTNRDI
70
ICQIAYARIE
80
GDMIVCAAYA
90
HELPKYGVKV
100
GLTNYAAAYC
110
TGLLLARRLL
120
NRFGMDKIYE
130
GQVEVNGGEY
140
NVESIDGQPG
150
AFTCYLDAGL
160
ARTTTGNKVF
170
GALKGAVDGG
180
LSIPHSTKRF
190
PGYDSESKEF
200
NAEVHRKHIM
210
GQNVADYMRY
220
LMEEDEDAYK
230
KQFSQYIKNN
240
VTPDMMEEMY
250
KKAHAAIREN
260
PVYEKKPKRE
270
VKKKRWNRPK
280
MSLAQKKDRV
290
AQKKASFLRA
QERAAES
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0017101 |
aminoacyl-tRNA synthetase multienzyme complex |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0022625 |
cytosolic large ribosomal subunit |
| Cellular Component |
GO:0022626 |
cytosolic ribosome |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0014069 |
postsynaptic density |
| Cellular Component |
GO:0098793 |
presynapse |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Cellular Component |
GO:1990904 |
ribonucleoprotein complex |
| Cellular Component |
GO:0005840 |
ribosome |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0008097 |
5S rRNA binding |
| Molecular Function |
GO:0003730 |
mRNA 3'-UTR binding |
| Molecular Function |
GO:0048027 |
mRNA 5'-UTR binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0003735 |
structural constituent of ribosome |
| Molecular Function |
GO:1990948 |
ubiquitin ligase inhibitor activity |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Biological Process |
GO:0002181 |
cytoplasmic translation |
| Biological Process |
GO:1901740 |
negative regulation of myoblast fusion |
| Biological Process |
GO:2000435 |
negative regulation of protein neddylation |
| Biological Process |
GO:2000059 |
negative regulation of ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:0010628 |
positive regulation of gene expression |
| Biological Process |
GO:0010922 |
positive regulation of phosphatase activity |
| Biological Process |
GO:0045727 |
positive regulation of translation |
| Biological Process |
GO:0050821 |
protein stabilization |
| Biological Process |
GO:1901796 |
regulation of signal transduction by p53 class mediator |
| Biological Process |
GO:0000027 |
ribosomal large subunit assembly |
| Biological Process |
GO:0042273 |
ribosomal large subunit biogenesis |
| Biological Process |
GO:0006364 |
rRNA processing |
| Biological Process |
GO:0007283 |
spermatogenesis |
| Biological Process |
GO:0006941 |
striated muscle contraction |
| Biological Process |
GO:0140242 |
translation at postsynapse |
| Biological Process |
GO:0140236 |
translation at presynapse |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.