Overview
| Uniprot ID | P47985 |
|---|
| Protein Name | Cytochrome b-c1 complex subunit Rieske, mitochondrial |
|---|
| Gene Name | UQCRFS1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 163 |
LSDIPEGKNMAFKWR |
Function
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed:31883641). The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. The Rieske protein is a catalytic core subunit containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2 conformational states during catalysis to transfer electrons from the quinol bound in the Q(0) site in cytochrome b to cytochrome c1 (By similarity). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (PubMed:28673544)
Protein Sequence
10
MLSVASRSGP
20
FAPVLSATSR
30
GVAGALRPLV
40
QATVPATPEQ
50
PVLDLKRPFL
60
SRESLSGQAV
70
RRPLVASVGL
80
NVPASVCYSH
90
TDIKVPDFSE
100
YRRLEVLDST
110
KSSRESSEAR
120
KGFSYLVTGV
130
TTVGVAYAAK
140
NAVTQFVSSM
150
SASADVLALA
160
KIEIKLSDIP
170
EGKNMAFKWR
180
GKPLFVRHRT
190
QKEIEQEAAV
200
ELSQLRDPQH
210
DLDRVKKPEW
220
VILIGVCTHL
230
GCVPIANAGD
240
FGGYYCPCHG
250
SHYDASGRIR
260
LGPAPLNLEV
270
PTYEFTSDDM
VIVG
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005743 |
mitochondrial inner membrane |
| Cellular Component |
GO:0005759 |
mitochondrial matrix |
| Cellular Component |
GO:0005739 |
mitochondrion |
| Cellular Component |
GO:0045275 |
respiratory chain complex III |
| Molecular Function |
GO:0051537 |
2 iron, 2 sulfur cluster binding |
| Molecular Function |
GO:0046872 |
metal ion binding |
| Molecular Function |
GO:0016491 |
oxidoreductase activity |
| Molecular Function |
GO:0008121 |
quinol-cytochrome-c reductase activity |
| Biological Process |
GO:0006122 |
mitochondrial electron transport, ubiquinol to cytochrome c |
| Biological Process |
GO:0034551 |
mitochondrial respiratory chain complex III assembly |
| Biological Process |
GO:0022904 |
respiratory electron transport chain |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.