Overview
| Uniprot ID | P48380 |
|---|
| Protein Name | Transcription factor RFX3 |
|---|
| Gene Name | RFX3 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 282 |
RQQPMQQKQRYKPMQ |
Function
Transcription factor required for ciliogenesis and islet cell differentiation during endocrine pancreas development. Essential for the differentiation of nodal monocilia and left-right asymmetry specification during embryogenesis. Required for the biogenesis of motile cilia by governing growth and beating efficiency of motile cells. Also required for ciliated ependymal cell differentiation. Regulates the expression of genes involved in ciliary assembly (DYNC2LI1, FOXJ1 and BBS4) and genes involved in ciliary motility (DNAH11, DNAH9 and DNAH5) (By similarity). Together with RFX6, participates in the differentiation of 4 of the 5 islet cell types during endocrine pancreas development, with the exception of pancreatic PP (polypeptide-producing) cells. Regulates transcription by forming a heterodimer with another RFX protein and binding to the X-box in the promoter of target genes (PubMed:20148032). Represses transcription of MAP1A in non-neuronal cells but not in neuronal cells (PubMed:12411430)
Protein Sequence
10
MQTSETGSDT
20
GSTVTLQTSV
30
ASQAAVPTQV
40
VQQVPVQQQV
50
QQVQTVQQVQ
60
HVYPAQVQYV
70
EGSDTVYTNG
80
AIRTTTYPYT
90
ETQMYSQNTG
100
GNYFDTQGSS
110
AQVTTVVSSH
120
SMVGTGGIQM
130
GVTGGQLISS
140
SGGTYLIGNS
150
MENSGHSVTH
160
TTRASPATIE
170
MAIETLQKSD
180
GLSTHRSSLL
190
NSHLQWLLDN
200
YETAEGVSLP
210
RSTLYNHYLR
220
HCQEHKLDPV
230
NAASFGKLIR
240
SIFMGLRTRR
250
LGTRGNSKYH
260
YYGIRVKPDS
270
PLNRLQEDMQ
280
YMAMRQQPMQ
290
QKQRYKPMQK
300
VDGVADGFTG
310
SGQQTGTSVE
320
QTVIAQSQHH
330
QQFLDASRAL
340
PEFGEVEISS
350
LPDGTTFEDI
360
KSLQSLYREH
370
CEAILDVVVN
380
LQFSLIEKLW
390
QTFWRYSPST
400
PTDGTTITES
410
SNLSEIESRL
420
PKAKLITLCK
430
HESILKWMCN
440
CDHGMYQALV
450
EILIPDVLRP
460
IPSALTQAIR
470
NFAKSLEGWL
480
SNAMNNIPQR
490
MIQTKVAAVS
500
AFAQTLRRYT
510
SLNHLAQAAR
520
AVLQNTSQIN
530
QMLSDLNRVD
540
FANVQEQASW
550
VCQCDDNMVQ
560
RLETDFKMTL
570
QQQSTLEQWA
580
AWLDNVMMQA
590
LKPYEGRPSF
600
PKAARQFLLK
610
WSFYSSMVIR
620
DLTLRSAASF
630
GSFHLIRLLY
640
DEYMFYLVEH
650
RVAQATGETP
660
IAVMGEFGDL
670
NAVSPGNLDK
680
DEGSEVESEM
690
DEELDDSSEP
700
QAKREKTELS
710
QAFPVGCMQP
720
VLETGVQPSL
730
LNPIHSEHIV
740
TSTQTIRQCS
ATGNTYTAV
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005576 |
extracellular region |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005667 |
transcription regulator complex |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003700 |
DNA-binding transcription factor activity |
| Molecular Function |
GO:0000981 |
DNA-binding transcription factor activity, RNA polymerase II-specific |
| Molecular Function |
GO:0000978 |
RNA polymerase II cis-regulatory region sequence-specific DNA binding |
| Molecular Function |
GO:1990837 |
sequence-specific double-stranded DNA binding |
| Molecular Function |
GO:0000976 |
transcription cis-regulatory region binding |
| Biological Process |
GO:0048469 |
cell maturation |
| Biological Process |
GO:0060271 |
cilium assembly |
| Biological Process |
GO:0060285 |
cilium-dependent cell motility |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0031018 |
endocrine pancreas development |
| Biological Process |
GO:0060287 |
epithelial cilium movement involved in determination of left/right asymmetry |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0045893 |
positive regulation of DNA-templated transcription |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
| Biological Process |
GO:2000078 |
positive regulation of type B pancreatic cell development |
| Biological Process |
GO:0006355 |
regulation of DNA-templated transcription |
| Biological Process |
GO:0050796 |
regulation of insulin secretion |
| Biological Process |
GO:0006357 |
regulation of transcription by RNA polymerase II |
| Biological Process |
GO:0072560 |
type B pancreatic cell maturation |
Reference
[1] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.