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Overview

Uniprot IDP48382
Protein NameDNA-binding protein RFX5
Gene NameRFX5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
541 QGDGTVSKGGRGPGS
564 KIPLVPSKVSVIKGS
569 PSKVSVIKGSRSQKE

Function

Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters

Protein Sequence

10 MAEDEPDAKS 20 PKTGGRAPPG 30 GAEAGEPTTL 40 LQRLRGTISK 50 AVQNKVEGIL 60 QDVQKFSDND 70 KLYLYLQLPS 80 GPTTGDKSSE 90 PSTLSNEEYM 100 YAYRWIRNHL 110 EEHTDTCLPK 120 QSVYDAYRKY 130 CESLACCRPL 140 STANFGKIIR 150 EIFPDIKARR 160 LGGRGQSKYC 170 YSGIRRKTLV 180 SMPPLPGLDL 190 KGSESPEMGP 200 EVTPAPRDEL 210 VEAACALTCD 220 WAERILKRSF 230 SSIVEVARFL 240 LQQHLISARS 250 AHAHVLKAMG 260 LAEEDEHAPR 270 ERSSKPKNGL 280 ENPEGGAHKK 290 PERLAQPPKD 300 LEARTGAGPL 310 ARGERKKSVV 320 ESSAPGANNL 330 QVNALVARLP 340 LLLPRAPRSL 350 IPPIPVSPPI 360 LAPRLSSGAL 370 KVATLPLSSR 380 AGAPPAAVPI 390 INMILPTVPA 400 LPGPGPGPGR 410 APPGGLTQPR 420 GTENREVGIG 430 GDQGPHDKGV 440 KRTAEVPVSE 450 ASGQAPPAKA 460 AKQDIEDTAS 470 DAKRKRGRPR 480 KKSGGSGERN 490 STPLKSAAAM 500 ESAQSSRLPW 510 ETWGSGGEGN 520 SAGGAERPGP 530 MGEAEKGAVL 540 AQGQGDGTVS 550 KGGRGPGSQH 560 TKEAEDKIPL 570 VPSKVSVIKG 580 SRSQKEAFPL 590 AKGEVDTAPQ 600 GNKDLKEHVL 610 QSSLSQEHKD PKATPP

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0090575 RNA polymerase II transcription regulator complex
Molecular Function GO:0001228 DNA-binding transcription activator activity, RNA polymerase II-specific
Molecular Function GO:0000981 DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0000977 RNA polymerase II transcription regulatory region sequence-specific DNA binding
Molecular Function GO:0043565 sequence-specific DNA binding
Molecular Function GO:1990837 sequence-specific double-stranded DNA binding
Molecular Function GO:0001221 transcription coregulator binding
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0045348 positive regulation of MHC class II biosynthetic process
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0006357 regulation of transcription by RNA polymerase II

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.