Search Results
Overview
| Uniprot ID | P48426 |
|---|---|
| Protein Name | Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha |
| Gene Name | PIP4K2A |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 145 | YDKRYIIKTITSEDV |
| 15 | GSSVLASKTKTKKKH |
| 228 | ASDKEKAKELPTLKD |
| 27 | KKHFVAQKVKLFRAS |
| 378 | AHAAKTVKHGAGAEI |
Function
Catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) (PubMed:23326584, PubMed:9367159). Has both ATP- and GTP-dependent kinase activities (PubMed:26774281). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2 (PubMed:18364242). May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation (By similarity). Required for lysosome-peroxisome membrane contacts and intracellular cholesterol transport through modulating peroxisomal PtdIns(4,5)P2 level (PubMed:29353240). In collaboration with PIP4K2B, has a role in mediating autophagy in times of nutrient stress (By similarity). Required for autophagosome-lysosome fusion and the regulation of cellular lipid metabolism (PubMed:31091439). May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size (By similarity). Negatively regulates insulin signaling through a catalytic-independent mechanism (PubMed:31091439). PIP4Ks interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3 (PubMed:31091439)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005776 | autophagosome |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0005764 | lysosome |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0001917 | photoreceptor inner segment |
| Cellular Component | GO:0001750 | photoreceptor outer segment |
| Cellular Component | GO:0005886 | plasma membrane |
| Molecular Function | GO:0016308 | 1-phosphatidylinositol-4-phosphate 5-kinase activity |
| Molecular Function | GO:0016309 | 1-phosphatidylinositol-5-phosphate 4-kinase activity |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0042803 | protein homodimerization activity |
| Biological Process | GO:1902635 | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process |
| Biological Process | GO:0061909 | autophagosome-lysosome fusion |
| Biological Process | GO:0035855 | megakaryocyte development |
| Biological Process | GO:0046627 | negative regulation of insulin receptor signaling pathway |
| Biological Process | GO:0046854 | phosphatidylinositol phosphate biosynthetic process |
| Biological Process | GO:2000786 | positive regulation of autophagosome assembly |
| Biological Process | GO:0010506 | regulation of autophagy |
| Biological Process | GO:0090119 | vesicle-mediated cholesterol transport |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.
[3] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.
[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.