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Overview

Uniprot IDP48500
Protein NameTriosephosphate isomerase
Gene NameTpi1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
142 REAGITEKVVFEQTK
149 KVVFEQTKAIADNVK
156 KAIADNVKDWCKVVL
188 QAQEVHEKLRGWLKC
194 EKLRGWLKCNVSEGV
59 ARQKLDPKIAVAAQN
6 **MAPSRKFFVGGNW

Function

Triosephosphate isomerase is an extremely efficient metabolic enzyme that catalyzes the interconversion between dihydroxyacetone phosphate (DHAP) and D-glyceraldehyde-3-phosphate (G3P) in glycolysis and gluconeogenesis

Protein Sequence

10 MAPSRKFFVG 20 GNWKMNGRKK 30 CLGELICTLN 40 AAKLPADTEV 50 VCAPPTAYID 60 FARQKLDPKI 70 AVAAQNCYKV 80 TNGAFTGEIS 90 PGMIKDLGAT 100 WVVLGHSERR 110 HIFGESDELI 120 GQKVNHALSE 130 GLGVIACIGE 140 KLDEREAGIT 150 EKVVFEQTKA 160 IADNVKDWCK 170 VVLAYEPVWA 180 IGTGKTATPQ 190 QAQEVHEKLR 200 GWLKCNVSEG 210 VAQCTRIIYG 220 GSVTGATCKE 230 LASQPDVDGF 240 LVGGASLKPE FVDIINAKQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Molecular Function GO:0016853 isomerase activity
Molecular Function GO:0008929 methylglyoxal synthase activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0004807 triose-phosphate isomerase activity
Molecular Function GO:0031625 ubiquitin protein ligase binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0006094 gluconeogenesis
Biological Process GO:0006006 glucose metabolic process
Biological Process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
Biological Process GO:0019682 glyceraldehyde-3-phosphate metabolic process
Biological Process GO:0019563 glycerol catabolic process
Biological Process GO:0006096 glycolytic process
Biological Process GO:0019242 methylglyoxal biosynthetic process

Reference

[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.

[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.