Search Results
Overview
| Uniprot ID | P48506 |
|---|---|
| Protein Name | Glutamate--cysteine ligase catalytic subunit |
| Gene Name | GCLC |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 492 | YFRKDICKGGNAVVD |
| 632 | KVKYSGSKTDSSN** |
Function
Catalyzes the ATP-dependent ligation of L-glutamate and L-cysteine and participates in the first and rate-limiting step in glutathione biosynthesis
Protein Sequence
10
MGLLSQGSPL
20
SWEETKRHAD
30
HVRRHGILQF
40
LHIYHAVKDR
50
HKDVLKWGDE
60
VEYMLVSFDH
70
ENKKVRLVLS
80
GEKVLETLQE
90
KGERTNPNHP
100
TLWRPEYGSY
110
MIEGTPGQPY
120
GGTMSEFNTV
130
EANMRKRRKE
140
ATSILEENQA
150
LCTITSFPRL
160
GCPGFTLPEV
170
KPNPVEGGAS
180
KSLFFPDEAI
190
NKHPRFSTLT
200
RNIRHRRGEK
210
VVINVPIFKD
220
KNTPSPFIET
230
FTEDDEASRA
240
SKPDHIYMDA
250
MGFGMGNCCL
260
QVTFQACSIS
270
EARYLYDQLA
280
TICPIVMALS
290
AASPFYRGYV
300
SDIDCRWGVI
310
SASVDDRTRE
320
ERGLEPLKNN
330
NYRISKSRYD
340
SIDSYLSKCG
350
EKYNDIDLTI
360
DKEIYEQLLQ
370
EGIDHLLAQH
380
VAHLFIRDPL
390
TLFEEKIHLD
400
DANESDHFEN
410
IQSTNWQTMR
420
FKPPPPNSDI
430
GWRVEFRPME
440
VQLTDFENSA
450
YVVFVVLLTR
460
VILSYKLDFL
470
IPLSKVDENM
480
KVAQKRDAVL
490
QGMFYFRKDI
500
CKGGNAVVDG
510
CGKAQNSTEL
520
AAEEYTLMSI
530
DTIINGKEGV
540
FPGLIPILNS
550
YLENMEVDVD
560
TRCSILNYLK
570
LIKKRASGEL
580
MTVARWMREF
590
IANHPDYKQD
600
SVITDEMNYS
610
LILKCNQIAN
620
ELCECPELLG
630
SAFRKVKYSG
SKTDSSN
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0017109 | glutamate-cysteine ligase complex |
| Molecular Function | GO:0043531 | ADP binding |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0016595 | glutamate binding |
| Molecular Function | GO:0004357 | glutamate-cysteine ligase activity |
| Molecular Function | GO:0000287 | magnesium ion binding |
| Molecular Function | GO:0044877 | protein-containing complex binding |
| Biological Process | GO:0097746 | blood vessel diameter maintenance |
| Biological Process | GO:0045454 | cell redox homeostasis |
| Biological Process | GO:0044344 | cellular response to fibroblast growth factor stimulus |
| Biological Process | GO:0071372 | cellular response to follicle-stimulating hormone stimulus |
| Biological Process | GO:0071333 | cellular response to glucose stimulus |
| Biological Process | GO:0035729 | cellular response to hepatocyte growth factor stimulus |
| Biological Process | GO:0032869 | cellular response to insulin stimulus |
| Biological Process | GO:0071260 | cellular response to mechanical stimulus |
| Biological Process | GO:0097069 | cellular response to thyroxine stimulus |
| Biological Process | GO:0006534 | cysteine metabolic process |
| Biological Process | GO:0006536 | glutamate metabolic process |
| Biological Process | GO:0006750 | glutathione biosynthetic process |
| Biological Process | GO:0043066 | negative regulation of apoptotic process |
| Biological Process | GO:0045892 | negative regulation of DNA-templated transcription |
| Biological Process | GO:2000490 | negative regulation of hepatic stellate cell activation |
| Biological Process | GO:0043524 | negative regulation of neuron apoptotic process |
| Biological Process | GO:0014823 | response to activity |
| Biological Process | GO:0046686 | response to cadmium ion |
| Biological Process | GO:0009408 | response to heat |
| Biological Process | GO:0009725 | response to hormone |
| Biological Process | GO:0044752 | response to human chorionic gonadotropin |
| Biological Process | GO:0070555 | response to interleukin-1 |
| Biological Process | GO:0051409 | response to nitrosative stress |
| Biological Process | GO:0007584 | response to nutrient |
| Biological Process | GO:0006979 | response to oxidative stress |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.