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Overview

Uniprot IDP49321
Protein NameNuclear autoantigenic sperm protein
Gene NameNASP
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
405 IERLTETKDGSGLEE
413 DGSGLEEKVRAKLVP
417 LEEKVRAKLVPSQEE
450 VAQGATEKSPEDKVQ
52 KLLGLGQKHLVMGDI
698 VSMIASRKPTDGASS

Function

Component of the histone chaperone network (PubMed:22195965). Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy (PubMed:22195965). Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA

Protein Sequence

10 MAMESTATAA 20 VAAELVSADK 30 IEDVPAPSTS 40 ADKVESLDVD 50 SEAKKLLGLG 60 QKHLVMGDIP 70 AAVNAFQEAA 80 SLLGKKYGET 90 ANECGEAFFF 100 YGKSLLELAR 110 MENGVLGNAL 120 EGVHVEEEEG 130 EKTEDESLVE 140 NNDNIDEEAR 150 EELREQVYDA 160 MGEKEEAKKT 170 EDKSLAKPET 180 DKEQDSEMEK 190 GGREDMDISK 200 SAEEPQEKVD 210 LTLDWLTETS 220 EEAKGGAAPE 230 GPNEAEVTSG 240 KPEQEVPDAE 250 EEKSVSGTDV 260 QEECREKGGQ 270 EKQGEVIVSI 280 EEKPKEVSEE 290 QPVVTLEKQG 300 TAVEVEAESL 310 DPTVKPVDVG 320 GDEPEEKVVT 330 SENEAGKAVL 340 EQLVGQEVPP 350 AEESPEVTTE 360 AAEASAVEAG 370 SEVSEKPGQE 380 APVLPKDGAV 390 NGPSVVGDQT 400 PIEPQTSIER 410 LTETKDGSGL 420 EEKVRAKLVP 430 SQEETKLSVE 440 ESEAAGDGVD 450 TKVAQGATEK 460 SPEDKVQIAA 470 NEETQEREEQ 480 MKEGEETEGS 490 EEDDKENDKT 500 EEMPNDSVLE 510 NKSLQENEEE 520 EIGNLELAWD 530 MLDLAKIIFK 540 RQETKEAQLY 550 AAQAHLKLGE 560 VSVESENYVQ 570 AVEEFQSCLN 580 LQEQYLEAHD 590 RLLAETHYQL 600 GLAYGYNSQY 610 DEAVAQFSKS 620 IEVIENRMAV 630 LNEQVKEAEG 640 SSAEYKKEIE 650 ELKELLPEIR 660 EKIEDAKESQ 670 RSGNVAELAL 680 KATLVESSTS 690 GFTPGGGGSS 700 VSMIASRKPT 710 DGASSSNCVT 720 DISHLVRKKR 730 KPEEESPRKD 740 DAKKAKQEPE 750 VNGGSGDAVP 760 SGNEVSENME 770 EEAENQAESR 780 AAVEGTVEAG ATVESTAC

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Molecular Function GO:0042393 histone binding
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0001824 blastocyst development
Biological Process GO:0034080 CENP-A containing chromatin assembly
Biological Process GO:0006260 DNA replication
Biological Process GO:0006335 DNA replication-dependent chromatin assembly
Biological Process GO:0008584 male gonad development
Biological Process GO:0006334 nucleosome assembly
Biological Process GO:0015031 protein transport
Biological Process GO:0033574 response to testosterone

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.