Search Results

Overview

Uniprot IDP49326
Protein NameFlavin-containing monooxygenase 5
Gene NameFMO5
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
168 FPGIEKFKGQYFHSR
186 NPEGFTGKRVIIIGI
255 CGQSLANKYLEKKIN
273 DHEMFGLKPKHRALS
275 EMFGLKPKHRALSQH
480 YRVQGPGKWDGARKA
486 GKWDGARKAILTTDD
57 EGRASIYKSVIINTS

Function

Acts as a Baeyer-Villiger monooxygenase on a broad range of substrates. Catalyzes the insertion of an oxygen atom into a carbon-carbon bond adjacent to a carbonyl, which converts ketones to esters (PubMed:20947616, PubMed:26771671, PubMed:28783300). Active on diverse carbonyl compounds, whereas soft nucleophiles are mostly non- or poorly reactive (PubMed:26771671, PubMed:7872795). In contrast with other forms of FMO it is non- or poorly active on 'classical' substrates such as drugs, pesticides, and dietary components containing soft nucleophilic heteroatoms (Probable) (PubMed:7872795). Able to oxidize drug molecules bearing a carbonyl group on an aliphatic chain, such as nabumetone and pentoxifylline (PubMed:28783300). Also, in the absence of substrates, shows slow but yet significant NADPH oxidase activity (PubMed:26771671). Acts as a positive modulator of cholesterol biosynthesis as well as glucose homeostasis, promoting metabolic aging via pleiotropic effects (By similarity)

Protein Sequence

10 MTKKRIAVIG 20 GGVSGLSSIK 30 CCVEEGLEPV 40 CFERTDDIGG 50 LWRFQENPEE 60 GRASIYKSVI 70 INTSKEMMCF 80 SDYPIPDHYP 90 NFMHNAQVLE 100 YFRMYAKEFD 110 LLKYIRFKTT 120 VCSVKKQPDF 130 ATSGQWEVVT 140 ESEGKKEMNV 150 FDGVMVCTGH 160 HTNAHLPLES 170 FPGIEKFKGQ 180 YFHSRDYKNP 190 EGFTGKRVII 200 IGIGNSGGDL 210 AVEISQTAKQ 220 VFLSTRRGAW 230 ILNRVGDYGY 240 PADVLFSSRL 250 THFIWKICGQ 260 SLANKYLEKK 270 INQRFDHEMF 280 GLKPKHRALS 290 QHPTLNDDLP 300 NRIISGLVKV 310 KGNVKEFTET 320 AAIFEDGSRE 330 DDIDAVIFAT 340 GYSFDFPFLE 350 DSVKVVKNKI 360 SLYKKVFPPN 370 LERPTLAIIG 380 LIQPLGAIMP 390 ISELQGRWAT 400 QVFKGLKTLP 410 SQSEMMAEIS 420 KAQEEIDKRY 430 VESQRHTIQG 440 DYIDTMEELA 450 DLVGVRPNLL 460 SLAFTDPKLA 470 LHLLLGPCTP 480 IHYRVQGPGK 490 WDGARKAILT 500 TDDRIRKPLM 510 TRVVERSSSM 520 TSTMTIGKFM 530 LALAFFAIII AYF

Gene Ontology

Classification GO ID Description
Molecular Function GO:0050660 flavin adenine dinucleotide binding
Molecular Function GO:0004497 monooxygenase activity
Molecular Function GO:0004499 N,N-dimethylaniline monooxygenase activity
Molecular Function GO:0016174 NAD(P)H oxidase H2O2-forming activity
Molecular Function GO:0050661 NADP binding
Biological Process GO:0006629 lipid metabolic process
Biological Process GO:0090181 regulation of cholesterol metabolic process
Biological Process GO:0006805 xenobiotic metabolic process
Cellular Component GO:0005783 endoplasmic reticulum
Cellular Component GO:0005789 endoplasmic reticulum membrane

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.