Overview
| Uniprot ID | P49336 |
|---|
| Protein Name | Cyclin-dependent kinase 8 |
|---|
| Gene Name | CDK8 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 118 |
HRASKANKKPVQLPR |
Function
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression (PubMed:10993082). Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation (PubMed:15546612). Selectively regulates the interferon response by phosphorylating the transcription factor STAT1 transactivation domain (By similarity)
Protein Sequence
10
MDYDFKVKLS
20
SERERVEDLF
30
EYEGCKVGRG
40
TYGHVYKAKR
50
KDGKDDKDYA
60
LKQIEGTGIS
70
MSACREIALL
80
RELKHPNVIS
90
LQKVFLSHAD
100
RKVWLLFDYA
110
EHDLWHIIKF
120
HRASKANKKP
130
VQLPRGMVKS
140
LLYQILDGIH
150
YLHANWVLHR
160
DLKPANILVM
170
GEGPERGRVK
180
IADMGFARLF
190
NSPLKPLADL
200
DPVVVTFWYR
210
APELLLGARH
220
YTKAIDIWAI
230
GCIFAELLTS
240
EPIFHCRQED
250
IKTSNPYHHD
260
QLDRIFNVMG
270
FPADKDWEDI
280
KKMPEHSTLM
290
KDFRRNTYTN
300
CSLIKYMEKH
310
KVKPDSKAFH
320
LLQKLLTMDP
330
IKRITSEQAM
340
QDPYFLEDPL
350
PTSDVFAGCQ
360
IPYPKREFLT
370
EEEPDDKGDK
380
KNQQQQQGNN
390
HTNGTGHPGN
400
QDSSHTQGPP
410
LKKVRVVPPT
420
TTSGGLIMTS
430
DYQRSNPHAA
440
YPNPGPSTSQ
450
PQSSMGYSAT
460
SQQPPQYSHQ
THRY
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:1990508 |
CKM complex |
| Cellular Component |
GO:0016592 |
mediator complex |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0004693 |
cyclin-dependent protein serine/threonine kinase activity |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0106310 |
protein serine kinase activity |
| Molecular Function |
GO:0008353 |
RNA polymerase II CTD heptapeptide repeat kinase activity |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
Reference
PMID: N/A