Search Results

Overview

Uniprot ID	P49591
Protein Name	Serine--tRNA ligase, cytoplasmic
Gene Name	SARS1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
12	LDLFRVDKGGDPALI
323	FRVHQFEKIEQFVYS

Function

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser) (PubMed:22353712, PubMed:24095058, PubMed:26433229, PubMed:28236339, PubMed:34570399, PubMed:36041817, PubMed:9431993). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) (PubMed:26433229, PubMed:28236339, PubMed:34570399, PubMed:9431993). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC (PubMed:24940000). Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA (PubMed:19423847, PubMed:19423848, PubMed:24940000)

Protein Sequence

10 MVLDLDLFRV 20 DKGGDPALIR 30 ETQEKRFKDP 40 GLVDQLVKAD 50 SEWRRCRFRA 60 DNLNKLKNLC 70 SKTIGEKMKK 80 KEPVGDDESV 90 PENVLSFDDL 100 TADALANLKV 110 SQIKKVRLLI 120 DEAILKCDAE 130 RIKLEAERFE 140 NLREIGNLLH 150 PSVPISNDED 160 VDNKVERIWG 170 DCTVRKKYSH 180 VDLVVMVDGF 190 EGEKGAVVAG 200 SRGYFLKGVL 210 VFLEQALIQY 220 ALRTLGSRGY 230 IPIYTPFFMR 240 KEVMQEVAQL 250 SQFDEELYKV 260 IGKGSEKSDD 270 NSYDEKYLIA 280 TSEQPIAALH 290 RDEWLRPEDL 300 PIKYAGLSTC 310 FRQEVGSHGR 320 DTRGIFRVHQ 330 FEKIEQFVYS 340 SPHDNKSWEM 350 FEEMITTAEE 360 FYQSLGIPYH 370 IVNIVSGSLN 380 HAASKKLDLE 390 AWFPGSGAFR 400 ELVSCSNCTD 410 YQARRLRIRY 420 GQTKKMMDKV 430 EFVHMLNATM 440 CATTRTICAI 450 LENYQTEKGI 460 TVPEKLKEFM 470 PPGLQELIPF 480 VKPAPIEQEP 490 SKKQKKQHEG 500 SKKKAAARDV 510 TLENRLQNME VTDA

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0060090	molecular adaptor activity
Molecular Function	GO:0042803	protein homodimerization activity
Molecular Function	GO:0000978	RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function	GO:0098619	selenocysteine-tRNA ligase activity
Molecular Function	GO:0004828	serine-tRNA ligase activity
Molecular Function	GO:0000049	tRNA binding
Biological Process	GO:0002181	cytoplasmic translation
Biological Process	GO:0006351	DNA-templated transcription
Biological Process	GO:0016525	negative regulation of angiogenesis
Biological Process	GO:0000122	negative regulation of transcription by RNA polymerase II
Biological Process	GO:1904046	negative regulation of vascular endothelial growth factor production
Biological Process	GO:0001514	selenocysteine incorporation
Biological Process	GO:0006434	seryl-tRNA aminoacylation
Biological Process	GO:0006412	translation
Biological Process	GO:0106217	tRNA C3-cytosine methylation
Biological Process	GO:0006400	tRNA modification

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.