Overview
| Uniprot ID | P49863 |
|---|
| Protein Name | Granzyme K |
|---|
| Gene Name | GZMK |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 211 |
KGQKDSCKGDSGGPL |
Function
Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:3262682, PubMed:39814882, PubMed:39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed:39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed:39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed:39814882, PubMed:39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed:24711407, PubMed:33271118, PubMed:35704599, PubMed:39614076, PubMed:39814882, PubMed:39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed:21760880, PubMed:26936634, PubMed:35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed:17308307)
Protein Sequence
10
MTKFSSFSLF
20
FLIVGAYMTH
30
VCFNMEIIGG
40
KEVSPHSRPF
50
MASIQYGGHH
60
VCGGVLIDPQ
70
WVLTAAHCQY
80
RFTKGQSPTV
90
VLGAHSLSKN
100
EASKQTLEIK
110
KFIPFSRVTS
120
DPQSNDIMLV
130
KLQTAAKLNK
140
HVKMLHIRSK
150
TSLRSGTKCK
160
VTGWGATDPD
170
SLRPSDTLRE
180
VTVTVLSRKL
190
CNSQSYYNGD
200
PFITKDMVCA
210
GDAKGQKDSC
220
KGDSGGPLIC
230
KGVFHAIVSG
240
GHECGVATKP
250
GIYTLLTKKY
260
QTWIKSNLVP
PHTN
Gene Ontology
| Classification |
GO ID |
Description |
| Molecular Function |
GO:0008236 |
serine-type peptidase activity |
| Cellular Component |
GO:0009986 |
cell surface |
| Cellular Component |
GO:0005615 |
extracellular space |
| Cellular Component |
GO:0106139 |
symbiont cell surface |
| Molecular Function |
GO:0005539 |
glycosaminoglycan binding |
| Molecular Function |
GO:0038187 |
pattern recognition receptor activity |
| Molecular Function |
GO:0004252 |
serine-type endopeptidase activity |
| Biological Process |
GO:0160257 |
complement activation, GZMK pathway |
| Biological Process |
GO:0140507 |
granzyme-mediated programmed cell death signaling pathway |
| Biological Process |
GO:0006954 |
inflammatory response |
| Biological Process |
GO:0008228 |
opsonization |
| Biological Process |
GO:0050729 |
positive regulation of inflammatory response |
| Biological Process |
GO:0090200 |
positive regulation of release of cytochrome c from mitochondria |
| Biological Process |
GO:0051604 |
protein maturation |
| Biological Process |
GO:0031638 |
zymogen activation |
Reference
PMID: N/A