Overview
| Uniprot ID | P49917 |
|---|
| Protein Name | DNA ligase 4 |
|---|
| Gene Name | LIG4 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 636 |
KAAPKMKKVIGIIEH |
Function
DNA ligase involved in DNA non-homologous end joining (NHEJ); required for double-strand break (DSB) repair and V(D)J recombination (PubMed:12517771, PubMed:17290226, PubMed:23523427, PubMed:29980672, PubMed:33586762, PubMed:8798671, PubMed:9242410, PubMed:9809069). Catalyzes the NHEJ ligation step of the broken DNA during DSB repair by resealing the DNA breaks after the gap filling is completed (PubMed:12517771, PubMed:17290226, PubMed:9242410, PubMed:9809069). Joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction (PubMed:12517771, PubMed:17290226, PubMed:9242410, PubMed:9809069). LIG4 is mechanistically flexible: it can ligate nicks as well as compatible DNA overhangs alone, while in the presence of XRCC4, it can ligate ends with 2-nucleotides (nt) microhomology and 1-nt gaps (PubMed:17290226). Forms a subcomplex with XRCC4; the LIG4-XRCC4 subcomplex is responsible for the NHEJ ligation step and XRCC4 enhances the joining activity of LIG4 (PubMed:9242410, PubMed:9809069). Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends (PubMed:10854421). LIG4 regulates nuclear localization of XRCC4 (PubMed:24984242)
Protein Sequence
10
MAASQTSQTV
20
ASHVPFADLC
30
STLERIQKSK
40
GRAEKIRHFR
50
EFLDSWRKFH
60
DALHKNHKDV
70
TDSFYPAMRL
80
ILPQLERERM
90
AYGIKETMLA
100
KLYIELLNLP
110
RDGKDALKLL
120
NYRTPTGTHG
130
DAGDFAMIAY
140
FVLKPRCLQK
150
GSLTIQQVND
160
LLDSIASNNS
170
AKRKDLIKKS
180
LLQLITQSSA
190
LEQKWLIRMI
200
IKDLKLGVSQ
210
QTIFSVFHND
220
AAELHNVTTD
230
LEKVCRQLHD
240
PSVGLSDISI
250
TLFSAFKPML
260
AAIADIEHIE
270
KDMKHQSFYI
280
ETKLDGERMQ
290
MHKDGDVYKY
300
FSRNGYNYTD
310
QFGASPTEGS
320
LTPFIHNAFK
330
ADIQICILDG
340
EMMAYNPNTQ
350
TFMQKGTKFD
360
IKRMVEDSDL
370
QTCYCVFDVL
380
MVNNKKLGHE
390
TLRKRYEILS
400
SIFTPIPGRI
410
EIVQKTQAHT
420
KNEVIDALNE
430
AIDKREEGIM
440
VKQPLSIYKP
450
DKRGEGWLKI
460
KPEYVSGLMD
470
ELDILIVGGY
480
WGKGSRGGMM
490
SHFLCAVAEK
500
PPPGEKPSVF
510
HTLSRVGSGC
520
TMKELYDLGL
530
KLAKYWKPFH
540
RKAPPSSILC
550
GTEKPEVYIE
560
PCNSVIVQIK
570
AAEIVPSDMY
580
KTGCTLRFPR
590
IEKIRDDKEW
600
HECMTLDDLE
610
QLRGKASGKL
620
ASKHLYIGGD
630
DEPQEKKRKA
640
APKMKKVIGI
650
IEHLKAPNLT
660
NVNKISNIFE
670
DVEFCVMSGT
680
DSQPKPDLEN
690
RIAEFGGYIV
700
QNPGPDTYCV
710
IAGSENIRVK
720
NIILSNKHDV
730
VKPAWLLECF
740
KTKSFVPWQP
750
RFMIHMCPST
760
KEHFAREYDC
770
YGDSYFIDTD
780
LNQLKEVFSG
790
IKNSNEQTPE
800
EMASLIADLE
810
YRYSWDCSPL
820
SMFRRHTVYL
830
DSYAVINDLS
840
TKNEGTRLAI
850
KALELRFHGA
860
KVVSCLAEGV
870
SHVIIGEDHS
880
RVADFKAFRR
890
TFKRKFKILK
900
ESWVTDSIDK
910
CELQEENQYL
I
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000781 |
chromosome, telomeric region |
| Cellular Component |
GO:0000793 |
condensed chromosome |
| Cellular Component |
GO:0032807 |
DNA ligase IV complex |
| Cellular Component |
GO:0005958 |
DNA-dependent protein kinase-DNA ligase 4 complex |
| Cellular Component |
GO:0070419 |
nonhomologous end joining complex |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0003677 |
DNA binding |
| Molecular Function |
GO:0003910 |
DNA ligase (ATP) activity |
| Molecular Function |
GO:0003909 |
DNA ligase activity |
| Molecular Function |
GO:0016874 |
ligase activity |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Biological Process |
GO:0006284 |
base-excision repair |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0008283 |
cell population proliferation |
| Biological Process |
GO:0071479 |
cellular response to ionizing radiation |
| Biological Process |
GO:0007417 |
central nervous system development |
| Biological Process |
GO:0051276 |
chromosome organization |
| Biological Process |
GO:0071897 |
DNA biosynthetic process |
| Biological Process |
GO:0006302 |
double-strand break repair |
| Biological Process |
GO:0097680 |
double-strand break repair via classical nonhomologous end joining |
| Biological Process |
GO:0006303 |
double-strand break repair via nonhomologous end joining |
| Biological Process |
GO:0075713 |
establishment of integrated proviral latency |
| Biological Process |
GO:0033152 |
immunoglobulin V(D)J recombination |
| Biological Process |
GO:0001701 |
in utero embryonic development |
| Biological Process |
GO:0045190 |
isotype switching |
| Biological Process |
GO:0043524 |
negative regulation of neuron apoptotic process |
| Biological Process |
GO:0051402 |
neuron apoptotic process |
| Biological Process |
GO:0006297 |
nucleotide-excision repair, DNA gap filling |
| Biological Process |
GO:2001252 |
positive regulation of chromosome organization |
| Biological Process |
GO:0048146 |
positive regulation of fibroblast proliferation |
| Biological Process |
GO:0050769 |
positive regulation of neurogenesis |
| Biological Process |
GO:0002328 |
pro-B cell differentiation |
| Biological Process |
GO:0010332 |
response to gamma radiation |
| Biological Process |
GO:0010165 |
response to X-ray |
| Biological Process |
GO:0000012 |
single strand break repair |
| Biological Process |
GO:0035019 |
somatic stem cell population maintenance |
| Biological Process |
GO:0033077 |
T cell differentiation in thymus |
| Biological Process |
GO:0033153 |
T cell receptor V(D)J recombination |
| Biological Process |
GO:0033151 |
V(D)J recombination |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.