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Overview

Uniprot IDP50137
Protein NameTransketolase
Gene NameTkt
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
16 QQKLQALKDTANRLR
232 CKAFGQAKHQPTAII
241 QPTAIIAKTFKGRGI
260 DKEAWHGKPLPKNMA
310 MPTPPNYKVGDKIAT
319 GDKIATRKAYGLALA
327 AYGLALAKLGHASDR
543 TIKPLDKKLILDCAR
59 FFHTMRYKALDPRNP
6 **MEGYHKPDQQKLQ

Function

Catalyzes the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor, via a covalent intermediate with the cofactor thiamine pyrophosphate (By similarity). Thus, catalyzes the reversible transfer of a two-carbon ketol group from sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate, producing xylulose-5-phosphate and ribose-5-phosphate (By similarity). The phosphate group of the substrate plays a role in stabilizing the substrate-thiamine intermediate while preventing the formation of the reactive enamine intermediate (By similarity). Can also use fructose-6-phosphate as a ketose donor (By similarity)

Protein Sequence

10 MEGYHKPDQQ 20 KLQALKDTAN 30 RLRISSIQAT 40 TAAGSGHPTS 50 CCSAAEIMAV 60 LFFHTMRYKA 70 LDPRNPHNDR 80 FVLSKGHAAP 90 ILYAVWAEAG 100 FLPEAELLNL 110 RKISSDLDGH 120 PVPKQAFTDV 130 ATGSLGQGLG 140 AACGMAYTGK 150 YFDKASYRVY 160 CMLGDGEVSE 170 GSVWEAMAFA 180 GIYKLDNLVA 190 IFDINRLGQS 200 DPAPLQHQVD 210 VYQKRCEAFG 220 WHAIIVDGHS 230 VEELCKAFGQ 240 AKHQPTAIIA 250 KTFKGRGITG 260 IEDKEAWHGK 270 PLPKNMAEQI 280 IQEIYSQVQS 290 KKKILATPPQ 300 EDAPSVDIAN 310 IRMPTPPNYK 320 VGDKIATRKA 330 YGLALAKLGH 340 ASDRIIALDG 350 DTKNSTFSEL 360 FKKEHPDRFI 370 ECYIAEQNMV 380 SIAVGCATRD 390 RTVPFCSTFA 400 AFFTRAFDQI 410 RMAAISESNI 420 NLCGSHCGVS 430 IGEDGPSQMA 440 LEDLAMFRSV 450 PMSTVFYPSD 460 GVATEKAVEL 470 AANTKGICFI 480 RTSRPENAII 490 YSNNEDFQVG 500 QAKVVLKSKD 510 DQVTVIGAGV 520 TLHEALAAAE 530 MLKKEKIGVR 540 VLDPFTIKPL 550 DKKLILDCAR 560 ATKGRILTVE 570 DHYYEGGIGE 580 AVSAVVVGEP 590 GVTVTRLAVS 600 QVPRSGKPAE 610 LLKMFGIDKD 620 AIVQAVKGLV TKG

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005789 endoplasmic reticulum membrane
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0005509 calcium ion binding
Molecular Function GO:0030246 carbohydrate binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0048029 monosaccharide binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0030976 thiamine pyrophosphate binding
Molecular Function GO:0004802 transketolase activity
Biological Process GO:1901159 D-xylulose 5-phosphate biosynthetic process
Biological Process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
Biological Process GO:0006098 pentose-phosphate shunt
Biological Process GO:0009052 pentose-phosphate shunt, non-oxidative branch
Biological Process GO:0040008 regulation of growth

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.