Search Results

Overview

Uniprot IDP50440
Protein NameGlycine amidinotransferase, mitochondrial
Gene NameGATM
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
102 VKANTYEKYWPFYQK
109 KYWPFYQKQGGHYFP
117 QGGHYFPKDHLKKAV
122 FPKDHLKKAVAEIEE
152 DPIDWSLKYKTPDFE
154 IDWSLKYKTPDFEST
201 RAYRSIIKDYFHRGA
209 DYFHRGAKWTTAPKP
237 HSVEDRHKLAAQGKF
332 HQIDLFKKAGWTIIT
381 ANEVPIQKMFEKLGI
385 PIQKMFEKLGITTIK
392 KLGITTIKVNIRNAN
55 NSCAADDKATEPLPK
96 PPFTIEVKANTYEKY

Function

Transamidinase that catalyzes the transfer of the amidino group of L-arginine onto the amino moiety of acceptor metabolites such as glycine, beta-alanine, gamma-aminobutyric acid (GABA) and taurine yielding the corresponding guanidine derivatives (PubMed:16820567, PubMed:27233232, PubMed:36543883, PubMed:3800397). Catalyzes the rate-limiting step of creatine biosynthesis, namely the transfer of the amidino group from L-arginine to glycine to generate guanidinoacetate, which is then methylated by GAMT to form creatine. Provides creatine as a source for ATP generation in tissues with high energy demands, in particular skeletal muscle, heart and brain (Probable) (PubMed:27233232, PubMed:36543883, PubMed:3800397, PubMed:9266688)

Protein Sequence

10 MLRVRCLRGG 20 SRGAEAVHYI 30 GSRLGRTLTG 40 WVQRTFQSTQ 50 AATASSRNSC 60 AADDKATEPL 70 PKDCPVSSYN 80 EWDPLEEVIV 90 GRAENACVPP 100 FTIEVKANTY 110 EKYWPFYQKQ 120 GGHYFPKDHL 130 KKAVAEIEEM 140 CNILKTEGVT 150 VRRPDPIDWS 160 LKYKTPDFES 170 TGLYSAMPRD 180 ILIVVGNEII 190 EAPMAWRSRF 200 FEYRAYRSII 210 KDYFHRGAKW 220 TTAPKPTMAD 230 ELYNQDYPIH 240 SVEDRHKLAA 250 QGKFVTTEFE 260 PCFDAADFIR 270 AGRDIFAQRS 280 QVTNYLGIEW 290 MRRHLAPDYR 300 VHIISFKDPN 310 PMHIDATFNI 320 IGPGIVLSNP 330 DRPCHQIDLF 340 KKAGWTIITP 350 PTPIIPDDHP 360 LWMSSKWLSM 370 NVLMLDEKRV 380 MVDANEVPIQ 390 KMFEKLGITT 400 IKVNIRNANS 410 LGGGFHCWTC 420 DVRRRGTLQS YLD

Gene Ontology

Classification GO ID Description
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005758 mitochondrial intermembrane space
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0015067 amidinotransferase activity
Molecular Function GO:0015068 glycine amidinotransferase activity
Biological Process GO:0006601 creatine biosynthetic process
Biological Process GO:0006600 creatine metabolic process
Biological Process GO:0007611 learning or memory
Biological Process GO:0014889 muscle atrophy
Biological Process GO:0120162 positive regulation of cold-induced thermogenesis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.