Search Results

Overview

Uniprot IDP50475
Protein NameAlanine--tRNA ligase, cytoplasmic
Gene NameAars1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
934 MDGKGGGKDMSAQAT

Function

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. In presence of high levels of lactate, also acts as a protein lactyltransferase that mediates lactylation of lysine residues in target proteins, such as TEAD1, TP53/p53 and YAP1. Protein lactylation takes place in a two-step reaction: lactate is first activated by ATP to form lactate-AMP and then transferred to lysine residues of target proteins. Acts as an inhibitor of TP53/p53 activity by catalyzing lactylation of TP53/p53. Acts as a positive regulator of the Hippo pathway by mediating lactylation of TEAD1 and YAP1

Protein Sequence

10 MDSTLTAREI 20 RERFINYFKR 30 NEHTYVHSSA 40 TIPLDDPTLL 50 FANAGMNQFK 60 PIFLNTVDPS 70 HPMAKLSRAA 80 NTQKCIRAGG 90 KHNDLDDVGK 100 DVYHHTFFEM 110 LGSWSFGDYF 120 KELACKMALE 130 LLTQEFGIPV 140 ERLYVTYFGG 150 DEAAGLEPDL 160 ECRQIWQNLG 170 LDEAKILPGN 180 MKDNFWEMGD 190 TGPCGPCSEI 200 HYDRIGGRDA 210 AHLVNQDDPN 220 VLEIWNLVFI 230 QYNRESDGVL 240 KPLPKKSIDT 250 GMGLERLVSV 260 LQNKMSNYDT 270 DLFVPYFEAI 280 QKGTGARPYT 290 GKVGAEDTDG 300 IDMAYRVLAD 310 HARTITVALA 320 DGGRPDNTGR 330 GYVLRRILRR 340 AVRYSHEKLN 350 ASRGFFATLV 360 DVVVQSLGDA 370 FPELKKDPDM 380 VKDIINEEEV 390 QFLKTLSRGR 400 RILDRKIQSL 410 GDCQTIPGDT 420 AWLLYDTYGF 430 PVDLTGLIAE 440 EKGLVVDMDG 450 FEEERKLAQL 460 KSQGKGAGGE 470 DLIMLDIYAI 480 EELRAKGLEA 490 TDDSPKYNYH 500 SDSSGSYVFE 510 CTVATVLALR 520 REKMFVDEVV 530 TGQECGVVLD 540 KTCFYAEQGG 550 QIFDEGYLVK 560 VDDSSEDKTE 570 FTVKNAQVRG 580 GYVLHIGTIY 590 GNLRVGDQVR 600 LFIDEPRRRP 610 VMSNHTATHI 620 LNFALRSVLG 630 DADQKGSLVA 640 PDRLRFDFTA 650 KGAMSTEQIK 660 KTEEIVNGMI 670 EAAKPVYTLD 680 CPLAAAKAIQ 690 GLRAVFDETY 700 PDPVRVVSIG 710 VPVSELLDDP 720 SGPAGSLTSV 730 EFCGGTHLRN 740 SSHAGAFVIV 750 TEEAIAKGIR 760 RIVAVTGAEA 770 QKALRKSETL 780 KKSLSAMEVK 790 VKAQSAPNKD 800 VQKEIADLGE 810 VLATAVIPQW 820 QKDEQRETLK 830 SLKKVMDDLD 840 RASKADVQKR 850 VLEKTKQLID 860 SNPNQPLVIL 870 EMESGASAKA 880 LNEALKLFKT 890 HSPQTSAMLF 900 TVDNEAGKIT 910 CLCQVPQNAA 920 NRGLKASEWV 930 QQVSGLMDGK 940 GGGKDMSAQA 950 TGKNVGCLQE 960 ALQLATSFAQ LRLGDVKN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005634 nucleus
Molecular Function GO:0004813 alanine-tRNA ligase activity
Molecular Function GO:0016597 amino acid binding
Molecular Function GO:0002161 aminoacyl-tRNA deacylase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0141207 peptide lactyltransferase (ATP-dependent) activity
Molecular Function GO:0002196 Ser-tRNA(Ala) deacylase activity
Molecular Function GO:0000049 tRNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006419 alanyl-tRNA aminoacylation
Biological Process GO:0021680 cerebellar Purkinje cell layer development
Biological Process GO:0043524 negative regulation of neuron apoptotic process
Biological Process GO:1901797 negative regulation of signal transduction by p53 class mediator
Biological Process GO:0050905 neuromuscular process
Biological Process GO:0050885 neuromuscular process controlling balance
Biological Process GO:0035332 positive regulation of hippo signaling
Biological Process GO:0140018 regulation of cytoplasmic translational fidelity
Biological Process GO:0006400 tRNA modification

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.