Search Results

Overview

Uniprot IDP50479
Protein NamePDZ and LIM domain protein 4
Gene NamePDLIM4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
206 EPVAAEPKQSGSFRY
236 PGGPRNLKPTASKLG
241 NLKPTASKLGAPLSG

Function

Suppresses SRC activation by recognizing and binding to active SRC and facilitating PTPN13-mediated dephosphorylation of SRC 'Tyr-419' leading to its inactivation. Inactivated SRC dissociates from this protein allowing the initiation of a new SRC inactivation cycle (PubMed:19307596). Involved in reorganization of the actin cytoskeleton (PubMed:21636573). In nonmuscle cells, binds to ACTN1 (alpha-actinin-1), increases the affinity of ACTN1 to F-actin (filamentous actin), and promotes formation of actin stress fibers. Involved in regulation of the synaptic AMPA receptor transport in dendritic spines of hippocampal pyramidal neurons directing the receptors toward an insertion at the postsynaptic membrane. Links endosomal surface-internalized GRIA1-containing AMPA receptors to the alpha-actinin/actin cytoskeleton. Increases AMPA receptor-mediated excitatory postsynaptic currents in neurons (By similarity)

Protein Sequence

10 MPHSVTLRGP 20 SPWGFRLVGG 30 RDFSAPLTIS 40 RVHAGSKAAL 50 AALCPGDLIQ 60 AINGESTELM 70 THLEAQNRIK 80 GCHDHLTLSV 90 SRPEGRSWPS 100 APDDSKAQAH 110 RIHIDPEIQD 120 GSPTTSRRPS 130 GTGTGPEDGR 140 PSLGSPYGQP 150 PRFPVPHNGS 160 SEATLPAQMS 170 TLHVSPPPSA 180 DPARGLPRSR 190 DCRVDLGSEV 200 YRMLREPAEP 210 VAAEPKQSGS 220 FRYLQGMLEA 230 GEGGDWPGPG 240 GPRNLKPTAS 250 KLGAPLSGLQ 260 GLPECTRCGH 270 GIVGTIVKAR 280 DKLYHPECFM 290 CSDCGLNLKQ 300 RGYFFLDERL 310 YCESHAKARV 320 KPPEGYDVVA 330 VYPNAKVELV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005912 adherens junction
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005856 cytoskeleton
Cellular Component GO:0005829 cytosol
Cellular Component GO:0043197 dendritic spine
Cellular Component GO:0031905 early endosome lumen
Cellular Component GO:0031901 early endosome membrane
Cellular Component GO:0031941 filamentous actin
Cellular Component GO:0030027 lamellipodium
Cellular Component GO:0005634 nucleus
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0045211 postsynaptic membrane
Cellular Component GO:0034777 recycling endosome lumen
Cellular Component GO:0055038 recycling endosome membrane
Cellular Component GO:0001725 stress fiber
Cellular Component GO:0030018 Z disc
Molecular Function GO:0003779 actin binding
Molecular Function GO:0051393 alpha-actinin binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0051371 muscle alpha-actinin binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0019903 protein phosphatase binding
Biological Process GO:0030036 actin cytoskeleton organization
Biological Process GO:0098976 excitatory chemical synaptic transmission
Biological Process GO:0007507 heart development
Biological Process GO:0061061 muscle structure development

Reference

[1] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.