Search Results

Overview

Uniprot IDP51003
Protein NamePoly(A) polymerase alpha
Gene NamePAPOLA
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
507 HVLQKKKKHSTEGVK
641 TSGNAATKIPTPIVG
650 PTPIVGVKRTSSPHK
740 PVIKNSIKLRLNR**

Function

Polymerase that creates the 3'-poly(A) tail of mRNA's. Also required for the endoribonucleolytic cleavage reaction at some polyadenylation sites. May acquire specificity through interaction with a cleavage and polyadenylation specificity factor (CPSF) at its C-terminus

Protein Sequence

10 MPFPVTTQGS 20 QQTQPPQKHY 30 GITSPISLAA 40 PKETDCVLTQ 50 KLIETLKPFG 60 VFEEEEELQR 70 RILILGKLNN 80 LVKEWIREIS 90 ESKNLPQSVI 100 ENVGGKIFTF 110 GSYRLGVHTK 120 GADIDALCVA 130 PRHVDRSDFF 140 TSFYDKLKLQ 150 EEVKDLRAVE 160 EAFVPVIKLC 170 FDGIEIDILF 180 ARLALQTIPE 190 DLDLRDDSLL 200 KNLDIRCIRS 210 LNGCRVTDEI 220 LHLVPNIDNF 230 RLTLRAIKLW 240 AKRHNIYSNI 250 LGFLGGVSWA 260 MLVARTCQLY 270 PNAIASTLVH 280 KFFLVFSKWE 290 WPNPVLLKQP 300 EECNLNLPVW 310 DPRVNPSDRY 320 HLMPIITPAY 330 PQQNSTYNVS 340 VSTRMVMVEE 350 FKQGLAITDE 360 ILLSKAEWSK 370 LFEAPNFFQK 380 YKHYIVLLAS 390 APTEKQRLEW 400 VGLVESKIRI 410 LVGSLEKNEF 420 ITLAHVNPQS 430 FPAPKENPDK 440 EEFRTMWVIG 450 LVFKKTENSE 460 NLSVDLTYDI 470 QSFTDTVYRQ 480 AINSKMFEVD 490 MKIAAMHVKR 500 KQLHQLLPNH 510 VLQKKKKHST 520 EGVKLTALND 530 SSLDLSMDSD 540 NSMSVPSPTS 550 ATKTSPLNSS 560 GSSQGRNSPA 570 PAVTAASVTN 580 IQATEVSVPQ 590 VNSSESSGGT 600 SSESIPQTAT 610 QPAISPPPKP 620 TVSRVVSSTR 630 LVNPPPRSSG 640 NAATSGNAAT 650 KIPTPIVGVK 660 RTSSPHKEES 670 PKKTKTEEDE 680 TSEDANCLAL 690 SGHDKTEAKE 700 QLDTETSTTQ 710 SETIQTAASL 720 LASQKTSSTD 730 LSDIPALPAN 740 PIPVIKNSIK LRLNR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0030145 manganese ion binding
Molecular Function GO:1990817 poly(A) RNA polymerase activity
Molecular Function GO:0003723 RNA binding
Biological Process GO:0180010 co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway
Biological Process GO:0031124 mRNA 3'-end processing

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.