Overview
| Uniprot ID | P51513 |
|---|
| Protein Name | RNA-binding protein Nova-1 |
|---|
| Gene Name | NOVA1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 53 |
EDGQYFLKVLIPSYA |
Function
RNA-binding protein which regulates alternative splicing of pre-mRNAs in the brain and spinal cord in a sequence-specific manner (By similarity). Binds to 5'-YCAY-3' repeats, with a minimum of 2 to 3 repeats necessary for high-affinity binding. Mediates both exon inclusion and exclusion, depending upon the position of its binding site within the pre-mRNA (PubMed:10811881). Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly. Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 small nuclear ribonucleoprotein (snRNP) binding and inhibiting exon inclusion, whereas binding to an intronic 5'-YCAY-3' repeat enhances spliceosome assembly and favors exon inclusion (By similarity). Regulates the splicing of gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2) and glycine receptor subunit alpha-2 (GLRA2) pre-mRNAs, among others (By similarity). Autoregulates its own splicing. Binds to its own exon 4 and directs its exclusion, thus leading to NOVA1 isoform 3 production (By similarity). May affect the splicing of many genes involved in vocal behavior (By similarity)
Protein Sequence
10
MMAAAPIQQN
20
GTHTGVPIDL
30
DPPDSRKRPL
40
EAPPEAGSTK
50
RTNTGEDGQY
60
FLKVLIPSYA
70
AGSIIGKGGQ
80
TIVQLQKETG
90
ATIKLSKSKD
100
FYPGTTERVC
110
LIQGTVEALN
120
AVHGFIAEKI
130
REMPQNVAKT
140
EPVSILQPQT
150
TVNPDRIKQT
160
LPSSPTTTKS
170
SPSDPMTTSR
180
ANQVKIIVPN
190
STAGLIIGKG
200
GATVKAVMEQ
210
SGAWVQLSQK
220
PDGINLQERV
230
VTVSGEPEQN
240
RKAVELIIQK
250
IQEDPQSGSC
260
LNISYANVTG
270
PVANSNPTGS
280
PYANTAEVLP
290
TAAAAAGLLG
300
HANLAGVAAF
310
PAVLSGFTGN
320
DLVAITSALN
330
TLASYGYNLN
340
TLGLGLSQAA
350
ATGALAAAAA
360
SANPAAAAAN
370
LLATYASEAS
380
ASGSTAGGTA
390
GTFALGSLAA
400
ATAATNGYFG
410
AASPLAASAI
420
LGTEKSTDGS
430
KDVVEIAVPE
440
NLVGAILGKG
450
GKTLVEYQEL
460
TGARIQISKK
470
GEFVPGTRNR
480
KVTITGTPAA
490
TQAAQYLITQ
500
RITYEQGVRA
ANPQKVG
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Molecular Function |
GO:0003730 |
mRNA 3'-UTR binding |
| Molecular Function |
GO:0003729 |
mRNA binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:1990825 |
sequence-specific mRNA binding |
| Biological Process |
GO:0000398 |
mRNA splicing, via spliceosome |
| Biological Process |
GO:0120163 |
negative regulation of cold-induced thermogenesis |
| Biological Process |
GO:0007399 |
nervous system development |
| Biological Process |
GO:0000381 |
regulation of alternative mRNA splicing, via spliceosome |
| Biological Process |
GO:0006396 |
RNA processing |
| Biological Process |
GO:0008380 |
RNA splicing |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.