Search Results

Overview

Uniprot IDP51532
Protein NameSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Gene NameSMARCA4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1024 LLTDGSEKDKKGKGG
1027 DGSEKDKKGKGGTKT
1131 LRLDGTTKAEDRGML
1237 QAGMFDQKSSSHERR
437 LETALNAKAYKRSKR
94 DPRYNQMKGMGMRSG

Function

ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:15075294, PubMed:29374058, PubMed:30339381, PubMed:32459350). Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (PubMed:20418909). Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). In brown adipose tissue, involved in the regulation of thermogenic genes expression (By similarity)

Protein Sequence

10 MSTPDPPLGG 20 TPRPGPSPGP 30 GPSPGAMLGP 40 SPGPSPGSAH 50 SMMGPSPGPP 60 SAGHPIPTQG 70 PGGYPQDNMH 80 QMHKPMESMH 90 EKGMSDDPRY 100 NQMKGMGMRS 110 GGHAGMGPPP 120 SPMDQHSQGY 130 PSPLGGSEHA 140 SSPVPASGPS 150 SGPQMSSGPG 160 GAPLDGADPQ 170 ALGQQNRGPT 180 PFNQNQLHQL 190 RAQIMAYKML 200 ARGQPLPDHL 210 QMAVQGKRPM 220 PGMQQQMPTL 230 PPPSVSATGP 240 GPGPGPGPGP 250 GPGPAPPNYS 260 RPHGMGGPNM 270 PPPGPSGVPP 280 GMPGQPPGGP 290 PKPWPEGPMA 300 NAAAPTSTPQ 310 KLIPPQPTGR 320 PSPAPPAVPP 330 AASPVMPPQT 340 QSPGQPAQPA 350 PMVPLHQKQS 360 RITPIQKPRG 370 LDPVEILQER 380 EYRLQARIAH 390 RIQELENLPG 400 SLAGDLRTKA 410 TIELKALRLL 420 NFQRQLRQEV 430 VVCMRRDTAL 440 ETALNAKAYK 450 RSKRQSLREA 460 RITEKLEKQQ 470 KIEQERKRRQ 480 KHQEYLNSIL 490 QHAKDFKEYH 500 RSVTGKIQKL 510 TKAVATYHAN 520 TEREQKKENE 530 RIEKERMRRL 540 MAEDEEGYRK 550 LIDQKKDKRL 560 AYLLQQTDEY 570 VANLTELVRQ 580 HKAAQVAKEK 590 KKKKKKKKAE 600 NAEGQTPAIG 610 PDGEPLDETS 620 QMSDLPVKVI 630 HVESGKILTG 640 TDAPKAGQLE 650 AWLEMNPGYE 660 VAPRSDSEES 670 GSEEEEEEEE 680 EEQPQAAQPP 690 TLPVEEKKKI 700 PDPDSDDVSE 710 VDARHIIENA 720 KQDVDDEYGV 730 SQALARGLQS 740 YYAVAHAVTE 750 RVDKQSALMV 760 NGVLKQYQIK 770 GLEWLVSLYN 780 NNLNGILADE 790 MGLGKTIQTI 800 ALITYLMEHK 810 RINGPFLIIV 820 PLSTLSNWAY 830 EFDKWAPSVV 840 KVSYKGSPAA 850 RRAFVPQLRS 860 GKFNVLLTTY 870 EYIIKDKHIL 880 AKIRWKYMIV 890 DEGHRMKNHH 900 CKLTQVLNTH 910 YVAPRRLLLT 920 GTPLQNKLPE 930 LWALLNFLLP 940 TIFKSCSTFE 950 QWFNAPFAMT 960 GEKVDLNEEE 970 TILIIRRLHK 980 VLRPFLLRRL 990 KKEVEAQLPE 1000 KVEYVIKCDM 1010 SALQRVLYRH 1020 MQAKGVLLTD 1030 GSEKDKKGKG 1040 GTKTLMNTIM 1050 QLRKICNHPY 1060 MFQHIEESFS 1070 EHLGFTGGIV 1080 QGLDLYRASG 1090 KFELLDRILP 1100 KLRATNHKVL 1110 LFCQMTSLMT 1120 IMEDYFAYRG 1130 FKYLRLDGTT 1140 KAEDRGMLLK 1150 TFNEPGSEYF 1160 IFLLSTRAGG 1170 LGLNLQSADT 1180 VIIFDSDWNP 1190 HQDLQAQDRA 1200 HRIGQQNEVR 1210 VLRLCTVNSV 1220 EEKILAAAKY 1230 KLNVDQKVIQ 1240 AGMFDQKSSS 1250 HERRAFLQAI 1260 LEHEEQDESR 1270 HCSTGSGSAS 1280 FAHTAPPPAG 1290 VNPDLEEPPL 1300 KEEDEVPDDE 1310 TVNQMIARHE 1320 EEFDLFMRMD 1330 LDRRREEARN 1340 PKRKPRLMEE 1350 DELPSWIIKD 1360 DAEVERLTCE 1370 EEEEKMFGRG 1380 SRHRKEVDYS 1390 DSLTEKQWLK 1400 AIEEGTLEEI 1410 EEEVRQKKSS 1420 RKRKRDSDAG 1430 SSTPTTSTRS 1440 RDKDDESKKQ 1450 KKRGRPPAEK 1460 LSPNPPNLTK 1470 KMKKIVDAVI 1480 KYKDSSSGRQ 1490 LSEVFIQLPS 1500 RKELPEYYEL 1510 IRKPVDFKKI 1520 KERIRNHKYR 1530 SLNDLEKDVM 1540 LLCQNAQTFN 1550 LEGSLIYEDS 1560 IVLQSVFTSV 1570 RQKIEKEDDS 1580 EGEESEEEEE 1590 GEEEGSESES 1600 RSVKVKIKLG 1610 RKEKAQDRLK 1620 GGRRRPSRGS 1630 RAKPVVSDDD 1640 SEEEQEEDRS GSGSEED

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0016020 membrane
Cellular Component GO:0071565 nBAF complex
Cellular Component GO:0071564 npBAF complex
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0016514 SWI/SNF complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0008094 ATP-dependent activity, acting on DNA
Molecular Function GO:0140658 ATP-dependent chromatin remodeler activity
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0070182 DNA polymerase binding
Molecular Function GO:0004386 helicase activity
Molecular Function GO:0042393 histone binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0050681 nuclear androgen receptor binding
Molecular Function GO:0140750 nucleosome array spacer activity
Molecular Function GO:0002039 p53 binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0030957 Tat protein binding
Molecular Function GO:0003713 transcription coactivator activity
Molecular Function GO:0001221 transcription coregulator binding
Molecular Function GO:0003714 transcription corepressor activity
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0031507 heterochromatin formation
Biological Process GO:0043923 host-mediated activation of viral transcription
Biological Process GO:0060766 negative regulation of androgen receptor signaling pathway
Biological Process GO:0045596 negative regulation of cell differentiation
Biological Process GO:0030308 negative regulation of cell growth
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0007399 nervous system development
Biological Process GO:0003407 neural retina development
Biological Process GO:0006337 nucleosome disassembly
Biological Process GO:0045597 positive regulation of cell differentiation
Biological Process GO:0008284 positive regulation of cell population proliferation
Biological Process GO:0120162 positive regulation of cold-induced thermogenesis
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:2000781 positive regulation of double-strand break repair
Biological Process GO:1902661 positive regulation of glucose mediated signaling pathway
Biological Process GO:1902895 positive regulation of miRNA transcription
Biological Process GO:0045663 positive regulation of myoblast differentiation
Biological Process GO:1901798 positive regulation of signal transduction by p53 class mediator
Biological Process GO:1902459 positive regulation of stem cell population maintenance
Biological Process GO:0045582 positive regulation of T cell differentiation
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:1901838 positive regulation of transcription of nucleolar large rRNA by RNA polymerase I
Biological Process GO:0030177 positive regulation of Wnt signaling pathway
Biological Process GO:0070316 regulation of G0 to G1 transition
Biological Process GO:2000045 regulation of G1/S transition of mitotic cell cycle
Biological Process GO:0030071 regulation of mitotic metaphase/anaphase transition
Biological Process GO:2000819 regulation of nucleotide-excision repair
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0001188 RNA polymerase I preinitiation complex assembly
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.