Search Results

Overview

Uniprot ID	P51881
Protein Name	ADP/ATP translocase 2
Gene Name	Slc25a5
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
105	IFLGGVDKRTQFWRY
147	RLAADVGKAGAEREF
155	AGAEREFKGLGDCLV
163	GLGDCLVKIYKSDGI
166	DCLVKIYKSDGIKGL
245	MMMQSGRKGTDIMYT
33	VAPIERVKLLLQVQH
43	LQVQHASKQITADKQ
52	ITADKQYKGIIDCVV
63	DCVVRIPKEQGVLSF
92	QALNFAFKDKYKQIF
96	FAFKDKYKQIFLGGV

Function

ADP:ATP antiporter that mediates import of ADP into the mitochondrial matrix for ATP synthesis, and export of ATP out to fuel the cell (PubMed:31341297). Cycles between the cytoplasmic-open state (c-state) and the matrix-open state (m-state): operates by the alternating access mechanism with a single substrate-binding site intermittently exposed to either the cytosolic (c-state) or matrix (m-state) side of the inner mitochondrial membrane (By similarity). In addition to its ADP:ATP antiporter activity, also involved in mitochondrial uncoupling and mitochondrial permeability transition pore (mPTP) activity (PubMed:31341297, PubMed:31489369). Plays a role in mitochondrial uncoupling by acting as a proton transporter: proton transport uncouples the proton flows via the electron transport chain and ATP synthase to reduce the efficiency of ATP production and cause mitochondrial thermogenesis (PubMed:31341297). Proton transporter activity is inhibited by ADP:ATP antiporter activity, suggesting that SLC25A5/ANT2 acts as a master regulator of mitochondrial energy output by maintaining a delicate balance between ATP production (ADP:ATP antiporter activity) and thermogenesis (proton transporter activity) (PubMed:31341297). Proton transporter activity requires free fatty acids as cofactor, but does not transport it (PubMed:31341297). Probably mediates mitochondrial uncoupling in tissues that do not express UCP1 (PubMed:31341297). Also plays a key role in mPTP opening, a non-specific pore that enables free passage of the mitochondrial membranes to solutes of up to 1.5 kDa, and which contributes to cell death (PubMed:31489369). It is however unclear if SLC25A5/ANT2 constitutes a pore-forming component of mPTP or regulates it (PubMed:31489369). Acts as a regulator of mitophagy independently of ADP:ATP antiporter activity: promotes mitophagy via interaction with TIMM44, leading to inhibit the presequence translocase TIMM23, thereby promoting stabilization of PINK1 (PubMed:31618756). As part of the mitotic spindle-associated MMXD complex it may play a role in chromosome segregation (By similarity)

Protein Sequence

10 MTDAAVSFAK 20 DFLAGGVAAA 30 ISKTAVAPIE 40 RVKLLLQVQH 50 ASKQITADKQ 60 YKGIIDCVVR 70 IPKEQGVLSF 80 WRGNLANVIR 90 YFPTQALNFA 100 FKDKYKQIFL 110 GGVDKRTQFW 120 RYFAGNLASG 130 GAAGATSLCF 140 VYPLDFARTR 150 LAADVGKAGA 160 EREFKGLGDC 170 LVKIYKSDGI 180 KGLYQGFNVS 190 VQGIIIYRAA 200 YFGIYDTAKG 210 MLPDPKNTHI 220 FISWMIAQSV 230 TAVAGLTSYP 240 FDTVRRRMMM 250 QSGRKGTDIM 260 YTGTLDCWRK 270 IARDEGSKAF 280 FKGAWSNVLR 290 GMGGAFVLVL YDEIKKYT

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0045121	membrane raft
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0042645	mitochondrial nucleoid
Cellular Component	GO:0005757	mitochondrial permeability transition pore complex
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0071817	MMXD complex
Cellular Component	GO:0043209	myelin sheath
Molecular Function	GO:0000295	adenine nucleotide transmembrane transporter activity
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0005471	ATP:ADP antiporter activity
Molecular Function	GO:0017077	oxidative phosphorylation uncoupler activity
Molecular Function	GO:0015078	proton transmembrane transporter activity
Molecular Function	GO:0031625	ubiquitin protein ligase binding
Biological Process	GO:1990845	adaptive thermogenesis
Biological Process	GO:0051503	adenine nucleotide transport
Biological Process	GO:0030183	B cell differentiation
Biological Process	GO:1990830	cellular response to leukemia inhibitory factor
Biological Process	GO:0007059	chromosome segregation
Biological Process	GO:0030218	erythrocyte differentiation
Biological Process	GO:0140021	mitochondrial ADP transmembrane transport
Biological Process	GO:1990544	mitochondrial ATP transmembrane transport
Biological Process	GO:1901029	negative regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
Biological Process	GO:0008284	positive regulation of cell population proliferation
Biological Process	GO:1901526	positive regulation of mitophagy
Biological Process	GO:0046902	regulation of mitochondrial membrane permeability

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.