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Overview

Uniprot ID	P51965
Protein Name	Ubiquitin-conjugating enzyme E2 E1
Gene Name	UBE2E1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
35	TPKKKESKVSMSKNS
40	ESKVSMSKNSKLLST
43	VSMSKNSKLLSTSAK

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination. Catalyzes monoubiquitination of other proteins in both an E3-dependent and E3-independent manner (PubMed:27237050)

Protein Sequence

10 MSDDDSRAST 20 SSSSSSSSNQ 30 QTEKETNTPK 40 KKESKVSMSK 50 NSKLLSTSAK 60 RIQKELADIT 70 LDPPPNCSAG 80 PKGDNIYEWR 90 STILGPPGSV 100 YEGGVFFLDI 110 TFTPEYPFKP 120 PKVTFRTRIY 130 HCNINSQGVI 140 CLDILKDNWS 150 PALTISKVLL 160 SICSLLTDCN 170 PADPLVGSIA 180 TQYMTNRAEH 190 DRMARQWTKR YAT

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0000151	ubiquitin ligase complex
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0042296	ISG15 transferase activity
Molecular Function	GO:0061631	ubiquitin conjugating enzyme activity
Molecular Function	GO:0004842	ubiquitin-protein transferase activity
Biological Process	GO:0032020	ISG15-protein conjugation
Biological Process	GO:0045944	positive regulation of transcription by RNA polymerase II
Biological Process	GO:0070936	protein K48-linked ubiquitination
Biological Process	GO:0006513	protein monoubiquitination
Biological Process	GO:0000209	protein polyubiquitination
Biological Process	GO:0016567	protein ubiquitination
Biological Process	GO:0006511	ubiquitin-dependent protein catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.