Search Results

Overview

Uniprot ID	P52306
Protein Name	Rap1 GTPase-GDP dissociation stimulator 1
Gene Name	RAP1GDS1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
314	QKLFEGGKGSVFQRV

Function

Acts as a GEF (guanine nucleotide exchange factor) for the Rho family of small GTP-binding proteins (G proteins) that stimulates the dissociation of GDP to enable subsequent binding of GTP (PubMed:11948427, PubMed:1549351, PubMed:20709748, PubMed:28630045, PubMed:30190425). Additionally, appears to chaperone the processing and/or trafficking of small GTPases containing a C-terminal polybasic region independently of GEF activity (PubMed:20709748, PubMed:21242305). Targets include RAP1A/RAP1B, RHOA, RHOB, RHOC, RAC1 and KRAS (PubMed:11948427, PubMed:1549351, PubMed:20709748, PubMed:24415755). Regulates mitochondrial dynamics by controlling RHOT function to promote mitochondrial fission during high calcium conditions (PubMed:27716788). Able to promote the Ca(2+) release from the endoplasmic reticulum via both inositol trisphosphate (Ins3P) and ryanodine sensitive receptors leading to a enhanced mitochondrial Ca(2+) uptake (PubMed:24349085)

Protein Sequence

10 MDNLSDTLKK 20 LKITAVDKTE 30 DSLEGCLDCL 40 LQALAQNNTE 50 TSEKIQASGI 60 LQLFASLLTP 70 QSSCKAKVAN 80 IIAEVAKNEF 90 MRIPCVDAGL 100 ISPLVQLLNS 110 KDQEVLLQTG 120 RALGNICYDS 130 HEGRSAVDQA 140 GGAQIVIDHL 150 RSLCSITDPA 160 NEKLLTVFCG 170 MLMNYSNEND 180 SLQAQLINMG 190 VIPTLVKLLG 200 IHCQNAALTE 210 MCLVAFGNLA 220 ELESSKEQFA 230 STNIAEELVK 240 LFKKQIEHDK 250 REMIFEVLAP 260 LAENDAIKLQ 270 LVEAGLVECL 280 LEIVQQKVDS 290 DKEDDITELK 300 TGSDLMVLLL 310 LGDESMQKLF 320 EGGKGSVFQR 330 VLSWIPSNNH 340 QLQLAGALAI 350 ANFARNDANC 360 IHMVDNGIVE 370 KLMDLLDRHV 380 EDGNVTVQHA 390 ALSALRNLAI 400 PVINKAKMLS 410 AGVTEAVLKF 420 LKSEMPPVQF 430 KLLGTLRMLI 440 DAQAEAAEQL 450 GKNVKLVERL 460 VEWCEAKDHA 470 GVMGESNRLL 480 SALIRHSKSK 490 DVIKTIVQSG 500 GIKHLVTMAT 510 SEHVIMQNEA 520 LVALALIAAL 530 ELGTAEKDLE 540 SAKLVQILHR 550 LLADERSAPE 560 IKYNSMVLIC 570 ALMGSECLHK 580 EVQDLAFLDV 590 VSKLRSHENK 600 SVAQQASLTE QRLTVES

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Molecular Function	GO:0005085	guanyl-nucleotide exchange factor activity
Biological Process	GO:0080120	CAAX-box protein maturation
Biological Process	GO:0031034	myosin filament assembly
Biological Process	GO:0032471	negative regulation of endoplasmic reticulum calcium ion concentration
Biological Process	GO:0051561	positive regulation of mitochondrial calcium ion concentration
Biological Process	GO:0034504	protein localization to nucleus
Biological Process	GO:0010821	regulation of mitochondrion organization
Biological Process	GO:0014829	vascular associated smooth muscle contraction

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.