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Overview

Uniprot IDP52480
Protein NamePyruvate kinase PKM
Gene NamePkm
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
115 VAVALDTKGPEIRTG
135 GTAEVELKKGATLKI
166 LDYKNICKVVEVGSK
247 VFASFIRKAADVHEV
270 KNIKIISKIENHEGV
322 GRCNRAGKPVICATQ
505 KARGFFKKGDVVIVL

Function

Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival

Protein Sequence

10 MPKPHSEAGT 20 AFIQTQQLHA 30 AMADTFLEHM 40 CRLDIDSAPI 50 TARNTGIICT 60 IGPASRSVEM 70 LKEMIKSGMN 80 VARLNFSHGT 90 HEYHAETIKN 100 VREATESFAS 110 DPILYRPVAV 120 ALDTKGPEIR 130 TGLIKGSGTA 140 EVELKKGATL 150 KITLDNAYME 160 KCDENILWLD 170 YKNICKVVEV 180 GSKIYVDDGL 190 ISLQVKEKGA 200 DFLVTEVENG 210 GSLGSKKGVN 220 LPGAAVDLPA 230 VSEKDIQDLK 240 FGVEQDVDMV 250 FASFIRKAAD 260 VHEVRKVLGE 270 KGKNIKIISK 280 IENHEGVRRF 290 DEILEASDGI 300 MVARGDLGIE 310 IPAEKVFLAQ 320 KMMIGRCNRA 330 GKPVICATQM 340 LESMIKKPRP 350 TRAEGSDVAN 360 AVLDGADCIM 370 LSGETAKGDY 380 PLEAVRMQHL 390 IAREAEAAIY 400 HLQLFEELRR 410 LAPITSDPTE 420 AAAVGAVEAS 430 FKCCSGAIIV 440 LTKSGRSAHQ 450 VARYRPRAPI 460 IAVTRNPQTA 470 RQAHLYRGIF 480 PVLCKDAVLN 490 AWAEDVDLRV 500 NLAMDVGKAR 510 GFFKKGDVVI 520 VLTGWRPGSG 530 FTNTMRVVPV P

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005929 cilium
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0043209 myelin sheath
Cellular Component GO:0005634 nucleus
Cellular Component GO:0001917 photoreceptor inner segment
Cellular Component GO:1902912 pyruvate kinase complex
Cellular Component GO:0005791 rough endoplasmic reticulum
Cellular Component GO:0097228 sperm principal piece
Molecular Function GO:0043531 ADP binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0035402 histone H3T11 kinase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0004715 non-membrane spanning protein tyrosine kinase activity
Molecular Function GO:0030955 potassium ion binding
Molecular Function GO:0046983 protein dimerization activity
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0004713 protein tyrosine kinase activity
Molecular Function GO:0004743 pyruvate kinase activity
Molecular Function GO:0070324 thyroid hormone binding
Molecular Function GO:0003713 transcription coactivator activity
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0032869 cellular response to insulin stimulus
Biological Process GO:0006096 glycolytic process
Biological Process GO:2000767 positive regulation of cytoplasmic translation
Biological Process GO:1903672 positive regulation of sprouting angiogenesis
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0012501 programmed cell death

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.