Search Results

Overview

Uniprot ID	P53367
Protein Name	Arfaptin-1
Gene Name	ARFIP1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
107	GGQRTQTKSGPVILA
146	TRQIISEKLGRGSRT
171	ILRDNKKKYENILKL

Function

Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network (PubMed:22981988). Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold (PubMed:22981988, PubMed:9038142). Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs (PubMed:22981988). In turn, ARFs induce fission (PubMed:22981988). Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells (PubMed:22981988)

Protein Sequence

10 MAQESPKNSA 20 AEIPVTSNGE 30 VDDSREHSFN 40 RDLKHSLPSG 50 LGLSETQITS 60 HGFDNTKEGV 70 IEAGAFQGSP 80 APPLPSVMSP 90 SRVAASRLAQ 100 QGSDLIVPAG 110 GQRTQTKSGP 120 VILADEIKNP 130 AMEKLELVRK 140 WSLNTYKCTR 150 QIISEKLGRG 160 SRTVDLELEA 170 QIDILRDNKK 180 KYENILKLAQ 190 TLSTQLFQMV 200 HTQRQLGDAF 210 ADLSLKSLEL 220 HEEFGYNADT 230 QKLLAKNGET 240 LLGAINFFIA 250 SVNTLVNKTI 260 EDTLMTVKQY 270 ESARIEYDAY 280 RTDLEELNLG 290 PRDANTLPKI 300 EQSQHLFQAH 310 KEKYDKMRND 320 VSVKLKFLEE 330 NKVKVLHNQL 340 VLFHNAIAAY 350 FAGNQKQLEQ 360 TLKQFHIKLK 370 TPGVDAPSWL EEQ

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0000139	Golgi membrane
Cellular Component	GO:0032588	trans-Golgi network membrane
Molecular Function	GO:0070273	phosphatidylinositol-4-phosphate binding
Molecular Function	GO:0005543	phospholipid binding
Molecular Function	GO:0019904	protein domain specific binding
Biological Process	GO:0006886	intracellular protein transport
Biological Process	GO:1905280	negative regulation of retrograde transport, endosome to Golgi
Biological Process	GO:0034315	regulation of Arp2/3 complex-mediated actin nucleation
Biological Process	GO:0050708	regulation of protein secretion

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.