Search Results

Overview

Uniprot ID	P53395
Protein Name	Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Gene Name	Dbt
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
196	SEVVGSGKDGRILKE
243	TFPTPIAKPPVFTGK

Function

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component

Protein Sequence

10 MAAARVLRTW 20 SQNAVRLTCV 30 RYFQTFNSAR 40 VLKPKCVCSV 50 GYPLFKYSQP 60 RHSLRTAAVL 70 QGQVVQFKLS 80 DIGEGIREVT 90 IKEWYVKEGD 100 TVSQFDSICE 110 VQSDKASVTI 120 TSRYDGVIKR 130 LYYNLDDIAY 140 VGKPLIDIET 150 EALKDSEEDV 160 VETPAVSHDE 170 HTHQEIKGQK 180 TLATPAVRRL 190 AMENNIKLSE 200 VVGSGKDGRI 210 LKEDILSFLE 220 KQTGAILPPS 230 PKSEITPPPP 240 QPKDRTFPTP 250 IAKPPVFTGK 260 DRTEPVTGFQ 270 KAMVKTMSAA 280 LKIPHFGYCD 290 EIDLTQLVKL 300 REELKPVALA 310 RGIKLSFMPF 320 FLKAASLGLL 330 QFPILNASVD 340 ENCQNITYKA 350 SHNIGIAMDT 360 ELGLIVPNVK 370 NVQVRSVFEI 380 AMELNRLQKL 390 GSSGQLGTTD 400 LTGGTFTLSN 410 IGSIGGTYAK 420 PVILPPEVAI 430 GALGAIKALP 440 RFDQKGDVYK 450 AQIMNVSWSA 460 DHRVIDGATM 470 SRFSNLWKSY 480 LENPAFMLLD LK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0160157	branched-chain alpha-ketoacid dehydrogenase complex
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0015630	microtubule cytoskeleton
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0042645	mitochondrial nucleoid
Cellular Component	GO:0005739	mitochondrion
Molecular Function	GO:0043754	dihydrolipoamide branched chain acyltransferase activity
Molecular Function	GO:0031625	ubiquitin protein ligase binding
Biological Process	GO:0120552	branched-chain alpha-keto acid decarboxylation to branched-chain acyl-CoA
Biological Process	GO:0009083	branched-chain amino acid catabolic process

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.