Overview
| Uniprot ID | P53990 |
|---|
| Protein Name | IST1 homolog |
|---|
| Gene Name | IST1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 309 |
PKPEASAKLPSRPAD |
Function
ESCRT-III-like protein involved in cytokinesis, nuclear envelope reassembly and endosomal tubulation (PubMed:19129479, PubMed:26040712, PubMed:28242692). Is required for efficient abscission during cytokinesis (PubMed:19129479). Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (PubMed:19129479, PubMed:19129480). During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing (PubMed:26040712). Recruited to the reforming nuclear envelope (NE) during anaphase by LEMD2 (PubMed:28242692). Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST (PubMed:23897888)
Protein Sequence
10
MLGSGFKAER
20
LRVNLRLVIN
30
RLKLLEKKKT
40
ELAQKARKEI
50
ADYLAAGKDE
60
RARIRVEHII
70
REDYLVEAME
80
ILELYCDLLL
90
ARFGLIQSMK
100
ELDSGLAESV
110
STLIWAAPRL
120
QSEVAELKIV
130
ADQLCAKYSK
140
EYGKLCRTNQ
150
IGTVNDRLMH
160
KLSVEAPPKI
170
LVERYLIEIA
180
KNYNVPYEPD
190
SVVMAEAPPG
200
VETDLIDVGF
210
TDDVKKGGPG
220
RGGSGGFTAP
230
VGGPDGTVPM
240
PMPMPMPSAN
250
TPFSYPLPKG
260
PSDFNGLPMG
270
TYQAFPNIHP
280
PQIPATPPSY
290
ESVDDINADK
300
NISSAQIVGP
310
GPKPEASAKL
320
PSRPADNYDN
330
FVLPELPSVP
340
DTLPTASAGA
350
STSASEDIDF
360
DDLSRRFEEL
KKKT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035578 |
azurophil granule lumen |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005793 |
endoplasmic reticulum-Golgi intermediate compartment |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005576 |
extracellular region |
| Cellular Component |
GO:0090543 |
Flemming body |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0005635 |
nuclear envelope |
| Molecular Function |
GO:0045296 |
cadherin binding |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0090541 |
MIT domain binding |
| Molecular Function |
GO:0019904 |
protein domain specific binding |
| Molecular Function |
GO:0044877 |
protein-containing complex binding |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0061640 |
cytoskeleton-dependent cytokinesis |
| Biological Process |
GO:1904903 |
ESCRT III complex disassembly |
| Biological Process |
GO:0045184 |
establishment of protein localization |
| Biological Process |
GO:0008104 |
intracellular protein localization |
| Biological Process |
GO:0061952 |
midbody abscission |
| Biological Process |
GO:0036258 |
multivesicular body assembly |
| Biological Process |
GO:0045862 |
positive regulation of proteolysis |
| Biological Process |
GO:0015031 |
protein transport |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.