Search Results

Overview

Uniprot IDP54132
Protein NameRecQ-like DNA helicase BLM
Gene NameBLM
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
187 VSTAQKSKKGKRNFF
188 STAQKSKKGKRNFFK
190 AQKSKKGKRNFFKAQ
195 KGKRNFFKAQLYTTN
24 SARTLNNKLSLSKPK
31 KLSLSKPKFSGFTFK
56 VTNVSVAKTPVLRNK
91 QRVKDFFKNAPAGQE

Function

ATP-dependent DNA helicase that unwinds double-stranded (ds)DNA in a 3'-5' direction (PubMed:24816114, PubMed:25901030, PubMed:9388193, PubMed:9765292). Participates in DNA replication and repair (PubMed:12019152, PubMed:21325134, PubMed:23509288, PubMed:34606619). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:21325134). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution (PubMed:25901030). Binds single-stranded DNA (ssDNA), forked duplex DNA and Holliday junction DNA (PubMed:20639533, PubMed:24257077, PubMed:25901030). Unwinds G-quadruplex DNA; unwinding occurs in the 3'-5' direction and requires a 3' single-stranded end of at least 7 nucleotides (PubMed:18426915, PubMed:9765292). Helicase activity is higher on G-quadruplex substrates than on duplex DNA substrates (PubMed:9765292). Telomeres, immunoglobulin heavy chain switch regions and rDNA are notably G-rich; formation of G-quadruplex DNA would block DNA replication and transcription (PubMed:18426915, PubMed:9765292). Negatively regulates sister chromatid exchange (SCE) (PubMed:25901030). Recruited by the KHDC3L-OOEP scaffold to DNA replication forks where it is retained by TRIM25 ubiquitination, it thereby promotes the restart of stalled replication forks (By similarity)

Protein Sequence

10 MAAVPQNNLQ 20 EQLERHSART 30 LNNKLSLSKP 40 KFSGFTFKKK 50 TSSDNNVSVT 60 NVSVAKTPVL 70 RNKDVNVTED 80 FSFSEPLPNT 90 TNQQRVKDFF 100 KNAPAGQETQ 110 RGGSKSLLPD 120 FLQTPKEVVC 130 TTQNTPTVKK 140 SRDTALKKLE 150 FSSSPDSLST 160 INDWDDMDDF 170 DTSETSKSFV 180 TPPQSHFVRV 190 STAQKSKKGK 200 RNFFKAQLYT 210 TNTVKTDLPP 220 PSSESEQIDL 230 TEEQKDDSEW 240 LSSDVICIDD 250 GPIAEVHINE 260 DAQESDSLKT 270 HLEDERDNSE 280 KKKNLEEAEL 290 HSTEKVPCIE 300 FDDDDYDTDF 310 VPPSPEEIIS 320 ASSSSSKCLS 330 TLKDLDTSDR 340 KEDVLSTSKD 350 LLSKPEKMSM 360 QELNPETSTD 370 CDARQISLQQ 380 QLIHVMEHIC 390 KLIDTIPDDK 400 LKLLDCGNEL 410 LQQRNIRRKL 420 LTEVDFNKSD 430 ASLLGSLWRY 440 RPDSLDGPME 450 GDSCPTGNSM 460 KELNFSHLPS 470 NSVSPGDCLL 480 TTTLGKTGFS 490 ATRKNLFERP 500 LFNTHLQKSF 510 VSSNWAETPR 520 LGKKNESSYF 530 PGNVLTSTAV 540 KDQNKHTASI 550 NDLERETQPS 560 YDIDNFDIDD 570 FDDDDDWEDI 580 MHNLAASKSS 590 TAAYQPIKEG 600 RPIKSVSERL 610 SSAKTDCLPV 620 SSTAQNINFS 630 ESIQNYTDKS 640 AQNLASRNLK 650 HERFQSLSFP 660 HTKEMMKIFH 670 KKFGLHNFRT 680 NQLEAINAAL 690 LGEDCFILMP 700 TGGGKSLCYQ 710 LPACVSPGVT 720 VVISPLRSLI 730 VDQVQKLTSL 740 DIPATYLTGD 750 KTDSEATNIY 760 LQLSKKDPII 770 KLLYVTPEKI 780 CASNRLISTL 790 ENLYERKLLA 800 RFVIDEAHCV 810 SQWGHDFRQD 820 YKRMNMLRQK 830 FPSVPVMALT 840 ATANPRVQKD 850 ILTQLKILRP 860 QVFSMSFNRH 870 NLKYYVLPKK 880 PKKVAFDCLE 890 WIRKHHPYDS 900 GIIYCLSRRE 910 CDTMADTLQR 920 DGLAALAYHA 930 GLSDSARDEV 940 QQKWINQDGC 950 QVICATIAFG 960 MGIDKPDVRF 970 VIHASLPKSV 980 EGYYQESGRA 990 GRDGEISHCL 1000 LFYTYHDVTR 1010 LKRLIMMEKD 1020 GNHHTRETHF 1030 NNLYSMVHYC 1040 ENITECRRIQ 1050 LLAYFGENGF 1060 NPDFCKKHPD 1070 VSCDNCCKTK 1080 DYKTRDVTDD 1090 VKSIVRFVQE 1100 HSSSQGMRNI 1110 KHVGPSGRFT 1120 MNMLVDIFLG 1130 SKSAKIQSGI 1140 FGKGSAYSRH 1150 NAERLFKKLI 1160 LDKILDEDLY 1170 INANDQAIAY 1180 VMLGNKAQTV 1190 LNGNLKVDFM 1200 ETENSSSVKK 1210 QKALVAKVSQ 1220 REEMVKKCLG 1230 ELTEVCKSLG 1240 KVFGVHYFNI 1250 FNTVTLKKLA 1260 ESLSSDPEVL 1270 LQIDGVTEDK 1280 LEKYGAEVIS 1290 VLQKYSEWTS 1300 PAEDSSPGIS 1310 LSSSRGPGRS 1320 AAEELDEEIP 1330 VSSHYFASKT 1340 RNERKRKKMP 1350 ASQRSKRRKT 1360 ASSGSKAKGG 1370 SATCRKISSK 1380 TKSSSIIGSS 1390 SASHTSQATS 1400 GANSKLGIMA 1410 PPKPINRPFL KPSYAFS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005694 chromosome
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0005657 replication fork
Molecular Function GO:0043138 3'-5' DNA helicase activity
Molecular Function GO:1905773 8-hydroxy-2'-deoxyguanosine DNA binding
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0008094 ATP-dependent activity, acting on DNA
Molecular Function GO:0000405 bubble DNA binding
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003678 DNA helicase activity
Molecular Function GO:1990814 DNA/DNA annealing activity
Molecular Function GO:0061749 forked DNA-dependent helicase activity
Molecular Function GO:0000400 four-way junction DNA binding
Molecular Function GO:0009378 four-way junction helicase activity
Molecular Function GO:0051880 G-quadruplex DNA binding
Molecular Function GO:0004386 helicase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0002039 p53 binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0003697 single-stranded DNA binding
Molecular Function GO:0061821 telomeric D-loop binding
Molecular Function GO:0061849 telomeric G-quadruplex DNA binding
Molecular Function GO:0000403 Y-form DNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0072757 cellular response to camptothecin
Biological Process GO:0072711 cellular response to hydroxyurea
Biological Process GO:0071479 cellular response to ionizing radiation
Biological Process GO:0006974 DNA damage response
Biological Process GO:0000729 DNA double-strand break processing
Biological Process GO:0032392 DNA geometric change
Biological Process GO:0006310 DNA recombination
Biological Process GO:0006281 DNA repair
Biological Process GO:0006260 DNA replication
Biological Process GO:0000724 double-strand break repair via homologous recombination
Biological Process GO:0007095 mitotic G2 DNA damage checkpoint signaling
Biological Process GO:0051782 negative regulation of cell division
Biological Process GO:0045910 negative regulation of DNA recombination
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0051259 protein complex oligomerization
Biological Process GO:0051260 protein homooligomerization
Biological Process GO:0000079 regulation of cyclin-dependent protein serine/threonine kinase activity
Biological Process GO:0031297 replication fork processing
Biological Process GO:0071139 resolution of DNA recombination intermediates
Biological Process GO:0010165 response to X-ray
Biological Process GO:0090656 t-circle formation
Biological Process GO:0000723 telomere maintenance
Biological Process GO:0032201 telomere maintenance via semi-conservative replication
Biological Process GO:0061820 telomeric D-loop disassembly
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0000800 lateral element
Cellular Component GO:0000228 nuclear chromosome
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0016605 PML body

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.