Search Results
Overview
| Uniprot ID | P54578 |
|---|---|
| Protein Name | Ubiquitin carboxyl-terminal hydrolase 14 |
| Gene Name | USP14 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 313 | QRNALYIKSSKISRL |
| 336 | VRFFYKEKESVNAKV |
| 342 | EKESVNAKVLKDVKF |
| 49 | ARQKVMVKGGTLKDD |
Function
Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins (PubMed:35145029). Ensures the regeneration of ubiquitin at the proteasome (PubMed:18162577, PubMed:28396413). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell (PubMed:18162577). Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (PubMed:19106094). Also serves as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1 (PubMed:19135427). Indispensable for synaptic development and function at neuromuscular junctions (NMJs) (By similarity). Plays a role in the innate immune defense against viruses by stabilizing the viral DNA sensor CGAS and thus inhibiting its autophagic degradation (PubMed:27666593). Inhibits OPTN-mediated selective autophagic degradation of KDM4D and thereby negatively regulates H3K9me2 and H3K9me3 (PubMed:35145029)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0009986 | cell surface |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0031410 | cytoplasmic vesicle |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0070062 | extracellular exosome |
| Cellular Component | GO:0098978 | glutamatergic synapse |
| Cellular Component | GO:0005886 | plasma membrane |
| Cellular Component | GO:0099523 | presynaptic cytosol |
| Cellular Component | GO:0000502 | proteasome complex |
| Molecular Function | GO:0004843 | cysteine-type deubiquitinase activity |
| Molecular Function | GO:0004197 | cysteine-type endopeptidase activity |
| Molecular Function | GO:0101005 | deubiquitinase activity |
| Molecular Function | GO:0004866 | endopeptidase inhibitor activity |
| Molecular Function | GO:0061578 | K63-linked deubiquitinase activity |
| Molecular Function | GO:0070628 | proteasome binding |
| Biological Process | GO:0006954 | inflammatory response |
| Biological Process | GO:0045087 | innate immune response |
| Biological Process | GO:1904293 | negative regulation of ERAD pathway |
| Biological Process | GO:2000059 | negative regulation of ubiquitin-dependent protein catabolic process |
| Biological Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process | GO:0071108 | protein K48-linked deubiquitination |
| Biological Process | GO:0050920 | regulation of chemotaxis |
| Biological Process | GO:0061136 | regulation of proteasomal protein catabolic process |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.
[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.