Overview
| Uniprot ID | P54612 |
|---|
| Protein Name | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform |
|---|
| Gene Name | PPP2R1A |
|---|
| Organism | Sus scrofa |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 107 |
EETVVRDKAVESLRA |
| 133 |
AHFVPLVKRLAGGDW |
| 163 |
PRVSSAVKAELRQYF |
| 188 |
VRRAAASKLGEFAKV |
| 272 |
DKFTELQKAVGPEIT |
| 307 |
AAASHKVKEFCENLS |
| 467 |
EAATSNLKKLVEKFG |
| 472 |
NLKKLVEKFGKEWAH |
| 542 |
NVRFNVAKSLQKIGP |
Function
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (By similarity). Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway (By similarity). Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability (By similarity). Part of the striatin-interacting phosphatase and kinase (STRIPAK) complexes. STRIPAK complexes have critical roles in protein (de)phosphorylation and are regulators of multiple signaling pathways including Hippo, MAPK, nuclear receptor and cytoskeleton remodeling. Different types of STRIPAK complexes are involved in a variety of biological processes such as cell growth, differentiation, apoptosis, metabolism and immune regulation (By similarity). Key mediator of a quality checkpoint during transcription elongation as part of the Integrator-PP2A (INTAC) complex (By similarity). The INTAC complex drives premature transcription termination of transcripts that are unfavorably configured for transcriptional elongation: within the INTAC complex, acts as a scaffolding subunit for PPP2CA, which catalyzes dephosphorylation of the C-terminal domain (CTD) of Pol II subunit POLR2A/RPB1 and SUPT5H/SPT5, thereby preventing transcriptional elongation (By similarity). Regulates the recruitment of the SKA complex to kinetochores (By similarity)
Protein Sequence
10
MAAADGDDSL
20
YPIAVLIDEL
30
RNEDVQLRLN
40
SIKKLSTIAL
50
ALGVERTRSE
60
LLPFLTDTIY
70
DEDEVLLALA
80
EQLGTFTTLV
90
GGPEYVHCLL
100
PPLESLATVE
110
ETVVRDKAVE
120
SLRAISHEHS
130
PSDLEAHFVP
140
LVKRLAGGDW
150
FTSRTSACGL
160
FSVCYPRVSS
170
AVKAELRQYF
180
RNLCSDDTPM
190
VRRAAASKLG
200
EFAKVLELDN
210
VKSEIIPMFS
220
NLASDEQDSV
230
RLLAVEACVN
240
IAQLLPQEDL
250
EALVMPTLRQ
260
AAEDKSWRVR
270
YMVADKFTEL
280
QKAVGPEITK
290
TDLVPAFQNL
300
MKDCEAEVRA
310
AASHKVKEFC
320
ENLSADCREN
330
VIMTQILPCI
340
KELVSDANQH
350
VKSALASVIM
360
GLSPILGKDN
370
TIEHLLPLFL
380
AQLKDECPEV
390
RLNIISNLDC
400
VNEVIGIRQL
410
SQSLLPAIVE
420
LAEDAKWRVR
430
LAIIEYMPLL
440
AGQLGVEFFD
450
EKLNSLCMAW
460
LVDHVYAIRE
470
AATSNLKKLV
480
EKFGKEWAHA
490
TIIPKVLAMS
500
GDPNYLHRMT
510
TLFCINVLSE
520
VCGQDITTKH
530
MLPTVLRMAG
540
DPVANVRFNV
550
AKSLQKIGPI
560
LDNSTLQSEV
570
KPILEKLTQD
580
QDVDVKYFAQ
EALTVLSLA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0000775 |
chromosome, centromeric region |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0030425 |
dendrite |
| Cellular Component |
GO:0090443 |
FAR/SIN/STRIPAK complex |
| Cellular Component |
GO:0016328 |
lateral plasma membrane |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0000159 |
protein phosphatase type 2A complex |
| Molecular Function |
GO:0019888 |
protein phosphatase regulator activity |
| Biological Process |
GO:0007059 |
chromosome segregation |
| Biological Process |
GO:0051754 |
meiotic sister chromatid cohesion, centromeric |
| Biological Process |
GO:0035331 |
negative regulation of hippo signaling |
| Biological Process |
GO:0051898 |
negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction |
| Biological Process |
GO:0051225 |
spindle assembly |
| Biological Process |
GO:0043029 |
T cell homeostasis |
Reference
[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.