Search Results

Overview

Uniprot ID	P55160
Protein Name	Nck-associated protein 1-like
Gene Name	NCKAP1L
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
313	EDLFSSLKGYGKRVA

Function

Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F-actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Within the WAVE2 complex, enables the cortical actin network to restrain excessive degranulation and granule release by T-cells (PubMed:32647003). Required for efficient T-lymphocyte and neutrophil migration (PubMed:32647003). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE-independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648). In T-cells, required for proper mechanistic target of rapamycin complex 2 (mTORC2)-dependent AKT phosphorylation, cell proliferation and cytokine secretion, including that of IL2 and TNF (PubMed:32647003)

Protein Sequence

10 MSLTSAYQHK 20 LAEKLTILND 30 RGQGVLIRMY 40 NIKKTCSDPK 50 SKPPFLLEKS 60 MEPSLKYINK 70 KFPNIDVRNS 80 TQHLGPVHRE 90 KAEIIRFLTN 100 YYQSFVDVME 110 FRDHVYELLN 120 TIDACQCHFD 130 INLNFDFTRS 140 YLDLIVTYTS 150 VILLLSRIED 160 RRILIGMYNC 170 AHEMLHGHGD 180 PSFARLGQMV 190 LEYDHPLKKL 200 TEEFGPHTKA 210 VSGALLSLHF 220 LFVRRNQGAE 230 QWRSAQLLSL 240 ISNPPAMINP 250 ANSDTMACEY 260 LSVEVMERWI 270 IIGFLLCHGC 280 LNSNSQCQKL 290 WKLCLQGSLY 300 ITLIREDVLQ 310 VHKVTEDLFS 320 SLKGYGKRVA 330 DIKESKEHVI 340 ANSGQFHCQR 350 RQFLRMAVKE 360 LETVLADEPG 370 LLGPKALFAF 380 MALSFIRDEV 390 TWLVRHTENV 400 TKTKTPEDYA 410 DSSIAELLFL 420 LEGIRSLVRR 430 HIKVIQQYHL 440 QYLARFDALV 450 LSDIIQNLSV 460 CPEEESIIMS 470 SFVSILSSLN 480 LKQVDNGEKF 490 EFSGLRLDWF 500 RLQAYTSVAK 510 APLHLHENPD 520 LAKVMNLIVF 530 HSRMLDSVEK 540 LLVETSDLST 550 FCFHLRIFEK 560 MFAMTLEESA 570 MLRYAIAFPL 580 ICAHFVHCTH 590 EMCPEEYPHL 600 KNHGLHHCNS 610 FLEELAKQTS 620 NCVLEICAEQ 630 RNLSEQLLPK 640 HCATTISKAK 650 NKKTRKQRQT 660 PRKGEPERDK 670 PGAESHRKNR 680 SIVTNMDKLH 690 LNLTELALTM 700 NHVYSFSVFE 710 HTIFPSEYLS 720 SHLEARLNRA 730 IVWLAGYNAT 740 TQEIVRPSEL 750 LAGVKAYIGF 760 IQSLAQFLGA 770 DASRVIRNAL 780 LQQTQPLDSC 790 GEQTITTLYT 800 NWYLESLLRQ 810 ASSGTIILSP 820 AMQAFVSLPR 830 EGEQNFSAEE 840 FSDISEMRAL 850 AELLGPYGMK 860 FLSENLMWHV 870 TSQIVELKKL 880 VVENMDILVQ 890 IRSNFSKPDL 900 MASLLPQLTG 910 AENVLKRMTI 920 IGVILSFRAM 930 AQEGLREVFS 940 SHCPFLMGPI 950 ECLKEFVTPD 960 TDIKVTLSIF 970 ELASAAGVGC 980 DIDPALVAAI 990 ANLKADTSSP 1000 EEEYKVACLL 1010 LIFLAVSLPL 1020 LATDPSSFYS 1030 IEKDGYNNNI 1040 HCLTKAIIQV 1050 SAALFTLYNK 1060 NIETHLKEFL 1070 VVASVSLLQL 1080 GQETDKLKTR 1090 NRESISLLMR 1100 LVVEESSFLT 1110 LDMLESCFPY 1120 VLLRNAYREV SRAFHLN

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Biological Process	GO:0065003	protein-containing complex assembly
Biological Process	GO:0009410	response to xenobiotic stimulus
Biological Process	GO:0043029	T cell homeostasis
Cellular Component	GO:0070062	extracellular exosome
Cellular Component	GO:0101003	ficolin-1-rich granule membrane
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0031209	SCAR complex
Cellular Component	GO:0030667	secretory granule membrane
Molecular Function	GO:0005096	GTPase activator activity
Molecular Function	GO:0030295	protein kinase activator activity
Molecular Function	GO:0044877	protein-containing complex binding
Molecular Function	GO:1904841	TORC2 complex binding
Biological Process	GO:0070358	actin polymerization-dependent cell motility
Biological Process	GO:0001782	B cell homeostasis
Biological Process	GO:0050853	B cell receptor signaling pathway
Biological Process	GO:0016477	cell migration
Biological Process	GO:0000902	cell morphogenesis
Biological Process	GO:0030031	cell projection assembly
Biological Process	GO:0006935	chemotaxis
Biological Process	GO:0030866	cortical actin cytoskeleton organization
Biological Process	GO:0048821	erythrocyte development
Biological Process	GO:0034101	erythrocyte homeostasis
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0030011	maintenance of cell polarity
Biological Process	GO:0002262	myeloid cell homeostasis
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0043318	negative regulation of cytotoxic T cell degranulation
Biological Process	GO:0032700	negative regulation of interleukin-17 production
Biological Process	GO:0032715	negative regulation of interleukin-6 production
Biological Process	GO:0048812	neuron projection morphogenesis
Biological Process	GO:0030838	positive regulation of actin filament polymerization
Biological Process	GO:0045579	positive regulation of B cell differentiation
Biological Process	GO:0030890	positive regulation of B cell proliferation
Biological Process	GO:0043372	positive regulation of CD4-positive, alpha-beta T cell differentiation
Biological Process	GO:0043378	positive regulation of CD8-positive, alpha-beta T cell differentiation
Biological Process	GO:0033630	positive regulation of cell adhesion mediated by integrin
Biological Process	GO:0045648	positive regulation of erythrocyte differentiation
Biological Process	GO:0045588	positive regulation of gamma-delta T cell differentiation
Biological Process	GO:0002687	positive regulation of leukocyte migration
Biological Process	GO:0045621	positive regulation of lymphocyte differentiation
Biological Process	GO:0090023	positive regulation of neutrophil chemotaxis
Biological Process	GO:1902624	positive regulation of neutrophil migration
Biological Process	GO:0060100	positive regulation of phagocytosis, engulfment
Biological Process	GO:0042102	positive regulation of T cell proliferation
Biological Process	GO:1904515	positive regulation of TORC2 signaling

Reference

[1] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.