Search Results

Overview

Uniprot IDP55196
Protein NameAfadin
Gene NameAFDN
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1042 IYVKSVVKGGAADVD
1337 TPASTLTKSGPGRWK
1807 QGQDVSNKVKASRKL
1813 NKVKASRKLTELENE
520 VDGGLMVKGPRHKPG

Function

Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs) (By similarity). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton (PubMed:11024295). May play a key role in the organization of epithelial structures of the embryonic ectoderm (By similarity). Essential for the organization of adherens junctions (PubMed:30463011)

Protein Sequence

10 MSAGGRDEER 20 RKLADIIHHW 30 NANRLDLFEI 40 SQPTEDLEFH 50 GVMRFYFQDK 60 AAGNFATKCI 70 RVSSTATTQD 80 VIETLAEKFR 90 PDMRMLSSPK 100 YSLYEVHVSG 110 ERRLDIDEKP 120 LVVQLNWNKD 130 DREGRFVLKN 140 ENDAIPPKKA 150 QSNGPEKQEK 160 EGVIQNFKRT 170 LSKKEKKEKK 180 KREKEALRQA 190 SDKDDRPFQG 200 EDVENSRLAA 210 EVYKDMPETS 220 FTRTISNPEV 230 VMKRRRQQKL 240 EKRMQEFRSS 250 DGRPDSGGTL 260 RIYADSLKPN 270 IPYKTILLST 280 TDPADFAVAE 290 ALEKYGLEKE 300 NPKDYCIARV 310 MLPPGAQHSD 320 EKGAKEIILD 330 DDECPLQIFR 340 EWPSDKGILV 350 FQLKRRPPDH 360 IPKKTKKHLE 370 GKTPKGKERA 380 DGSGYGSTLP 390 PEKLPYLVEL 400 SPGRRNHFAY 410 YNYHTYEDGS 420 DSRDKPKLYR 430 LQLSVTEVGT 440 EKLDDNSIQL 450 FGPGIQPHHC 460 DLTNMDGVVT 470 VTPRSMDAET 480 YVEGQRISET 490 TMLQSGMKVQ 500 FGASHVFKFV 510 DPSQDHALAK 520 RSVDGGLMVK 530 GPRHKPGIVQ 540 ETTFDLGGDI 550 HSGTALPTSK 560 STTRLDSDRV 570 SSASSTAERG 580 MVKPMIRVEQ 590 QPDYRRQESR 600 TQDASGPELI 610 LPASIEFRES 620 SEDSFLSAII 630 NYTNSSTVHF 640 KLSPTYVLYM 650 ACRYVLSNQY 660 RPDISPTERT 670 HKVIAVVNKM 680 VSMMEGVIQK 690 QKNIAGALAF 700 WMANASELLN 710 FIKQDRDLSR 720 ITLDAQDVLA 730 HLVQMAFKYL 740 VHCLQSELNN 750 YMPAFLDDPE 760 ENSLQRPKID 770 DVLHTLTGAM 780 SLLRRCRVNA 790 ALTIQLFSQL 800 FHFINMWLFN 810 RLVTDPDSGL 820 CSHYWGAIIR 830 QQLGHIEAWA 840 EKQGLELAAD 850 CHLSRIVQAT 860 TLLTMDKYAP 870 DDIPNINSTC 880 FKLNSLQLQA 890 LLQNYHCAPD 900 EPFIPTDLIE 910 NVVTVAENTA 920 DELARSDGRE 930 VQLEEDPDLQ 940 LPFLLPEDGY 950 SCDVVRNIPN 960 GLQEFLDPLC 970 QRGFCRLIPH 980 TRSPGTWTIY 990 FEGADYESHL 1000 LRENTELAQP 1010 LRKEPEIITV 1020 TLKKQNGMGL 1030 SIVAAKGAGQ 1040 DKLGIYVKSV 1050 VKGGAADVDG 1060 RLAAGDQLLS 1070 VDGRSLVGLS 1080 QERAAELMTR 1090 TSSVVTLEVA 1100 KQGAIYHGLA 1110 TLLNQPSPMM 1120 QRISDRRGSG 1130 KPRPKSEGFE 1140 LYNNSTQNGS 1150 PESPQLPWAE 1160 YSEPKKLPGD 1170 DRLMKNRADH 1180 RSSPNVANQP 1190 PSPGGKSAYA 1200 SGTTAKITSV 1210 STGNLCTEEQ 1220 TPPPRPEAYP 1230 IPTQTYTREY 1240 FTFPASKSQD 1250 RMAPPQNQWP 1260 NYEEKPHMHT 1270 DSNHSSIAIQ 1280 RVTRSQEELR 1290 EDKAYQLERH 1300 RIEAAMDRKS 1310 DSDMWINQSS 1320 SLDSSTSSQE 1330 HLNHSSKSVT 1340 PASTLTKSGP 1350 GRWKTPAAIP 1360 ATPVAVSQPI 1370 RTDLPPPPPP 1380 PPVHYAGDFD 1390 GMSMDLPLPP 1400 PPSANQIGLP 1410 SAQVAAAERR 1420 KREEHQRWYE 1430 KEKARLEEER 1440 ERKRREQERK 1450 LGQMRTQSLN 1460 PAPFSPLTAQ 1470 QMKPEKPSTL 1480 QRPQETVIRE 1490 LQPQQQPRTI 1500 ERRDLQYITV 1510 SKEELSSGDS 1520 LSPDPWKRDA 1530 KEKLEKQQQM 1540 HIVDMLSKEI 1550 QELQSKPDRS 1560 AEESDRLRKL 1570 MLEWQFQKRL 1580 QESKQKDEDD 1590 EEEEDDDVDT 1600 MLIMQRLEAE 1610 RRARLQDEER 1620 RRQQQLEEMR 1630 KREAEDRARQ 1640 EEERRRQEEE 1650 RTKRDAEEKR 1660 RQEEGYYSRL 1670 EAERRRQHDE 1680 AARRLLEPEA 1690 PGLCRPPLPR 1700 DYEPPSPSPA 1710 PGAPPPPPQR 1720 NASYLKTQVL 1730 SPDSLFTAKF 1740 VAYNEEEEEE 1750 DCSLAGPNSY 1760 PGSTGAAVGA 1770 HDACRDAKEK 1780 RSKSQDADSP 1790 GSSGAPENLT 1800 FKERQRLFSQ 1810 GQDVSNKVKA 1820 SRKLTELENE LNTK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005912 adherens junction
Cellular Component GO:0030054 cell junction
Cellular Component GO:0044291 cell-cell contact zone
Cellular Component GO:0005911 cell-cell junction
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0046930 pore complex
Cellular Component GO:0070160 tight junction
Molecular Function GO:0051015 actin filament binding
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0050839 cell adhesion molecule binding
Molecular Function GO:0031267 small GTPase binding
Biological Process GO:0070830 bicellular tight junction assembly
Biological Process GO:0007155 cell adhesion
Biological Process GO:0044331 cell-cell adhesion mediated by cadherin
Biological Process GO:0007267 cell-cell signaling
Biological Process GO:0090557 establishment of endothelial intestinal barrier
Biological Process GO:0061951 establishment of protein localization to plasma membrane
Biological Process GO:0030336 negative regulation of cell migration
Biological Process GO:0046931 pore complex assembly
Biological Process GO:0022409 positive regulation of cell-cell adhesion
Biological Process GO:2000049 positive regulation of cell-cell adhesion mediated by cadherin
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:0032880 regulation of protein localization
Biological Process GO:0007165 signal transduction

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Chao L, Xu Y, Yang Y, Ao X, Liang J. Identification of lactylation-related biomarkers for diagnosis, prognosis, and treatment responsiveness in triple-negative breast cancer.. World J Surg Oncol 24(1):77. 2026 Jan 22. PMID: 41566505.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.