Search Results

Overview

Uniprot IDP55786
Protein NamePuromycin-sensitive aminopeptidase
Gene NameNPEPPS
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
261 DFVETRSKDGVCVRV
274 RVYTPVGKAEQGKFA
863 VEGFAVDKMAGEVKA
913 HQYLLQRKASPPTV*

Function

Aminopeptidase with broad substrate specificity for several peptides. Involved in proteolytic events essential for cell growth and viability. May act as regulator of neuropeptide activity. Plays a role in the antigen-processing pathway for MHC class I molecules. Involved in the N-terminal trimming of cytotoxic T-cell epitope precursors. Digests the poly-Q peptides found in many cellular proteins. Digests tau from normal brain more efficiently than tau from Alzheimer disease brain

Protein Sequence

10 MWLAAAAPSL 20 ARRLLFLGPP 30 PPPLLLLVFS 40 RSSRRRLHSL 50 GLAAMPEKRP 60 FERLPADVSP 70 INYSLCLKPD 80 LLDFTFEGKL 90 EAAAQVRQAT 100 NQIVMNCADI 110 DIITASYAPE 120 GDEEIHATGF 130 NYQNEDEKVT 140 LSFPSTLQTG 150 TGTLKIDFVG 160 ELNDKMKGFY 170 RSKYTTPSGE 180 VRYAAVTQFE 190 ATDARRAFPC 200 WDEPAIKATF 210 DISLVVPKDR 220 VALSNMNVID 230 RKPYPDDENL 240 VEVKFARTPV 250 MSTYLVAFVV 260 GEYDFVETRS 270 KDGVCVRVYT 280 PVGKAEQGKF 290 ALEVAAKTLP 300 FYKDYFNVPY 310 PLPKIDLIAI 320 ADFAAGAMEN 330 WGLVTYRETA 340 LLIDPKNSCS 350 SSRQWVALVV 360 GHELAHQWFG 370 NLVTMEWWTH 380 LWLNEGFASW 390 IEYLCVDHCF 400 PEYDIWTQFV 410 SADYTRAQEL 420 DALDNSHPIE 430 VSVGHPSEVD 440 EIFDAISYSK 450 GASVIRMLHD 460 YIGDKDFKKG 470 MNMYLTKFQQ 480 KNAATEDLWE 490 SLENASGKPI 500 AAVMNTWTKQ 510 MGFPLIYVEA 520 EQVEDDRLLR 530 LSQKKFCAGG 540 SYVGEDCPQW 550 MVPITISTSE 560 DPNQAKLKIL 570 MDKPEMNVVL 580 KNVKPDQWVK 590 LNLGTVGFYR 600 TQYSSAMLES 610 LLPGIRDLSL 620 PPVDRLGLQN 630 DLFSLARAGI 640 ISTVEVLKVM 650 EAFVNEPNYT 660 VWSDLSCNLG 670 ILSTLLSHTD 680 FYEEIQEFVK 690 DVFSPIGERL 700 GWDPKPGEGH 710 LDALLRGLVL 720 GKLGKAGHKA 730 TLEEARRRFK 740 DHVEGKQILS 750 ADLRSPVYLT 760 VLKHGDGTTL 770 DIMLKLHKQA 780 DMQEEKNRIE 790 RVLGATLLPD 800 LIQKVLTFAL 810 SEEVRPQDTV 820 SVIGGVAGGS 830 KHGRKAAWKF 840 IKDNWEELYN 850 RYQGGFLISR 860 LIKLSVEGFA 870 VDKMAGEVKA 880 FFESHPAPSA 890 ERTIQQCCEN 900 ILLNAAWLKR 910 DAESIHQYLL QRKASPPTV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0005634 nucleus
Molecular Function GO:0016285 alanyl aminopeptidase activity
Molecular Function GO:0004177 aminopeptidase activity
Molecular Function GO:0070006 metalloaminopeptidase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0071456 cellular response to hypoxia
Biological Process GO:0043171 peptide catabolic process
Biological Process GO:1903955 positive regulation of protein targeting to mitochondrion
Biological Process GO:0000209 protein polyubiquitination
Biological Process GO:0006508 proteolysis

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.