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Overview

Uniprot IDP55809
Protein NameSuccinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial
Gene NameOXCT1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
173 QEGGSPIKYNKDGSV
176 GSPIKYNKDGSVAIA
185 GSVAIASKPREVREF
446 VTMEHSAKGNAHKIM
455 NAHKIMEKCTLPLTG
463 CTLPLTGKQCVNRII
473 VNRIITEKAVFDVDK
480 KAVFDVDKKKGLTLI

Function

Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production (PubMed:10964512). Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate (By similarity)

Protein Sequence

10 MAALKLLSSG 20 LRLCASARGS 30 GATWYKGCVC 40 SFSTSAHRHT 50 KFYTDPVEAV 60 KDIPDGATVL 70 VGGFGLCGIP 80 ENLIDALLKT 90 GVKGLTAVSN 100 NAGVDNFGLG 110 LLLRSKQIKR 120 MVSSYVGENA 130 EFERQYLSGE 140 LEVELTPQGT 150 LAERIRAGGA 160 GVPAFYTPTG 170 YGTLVQEGGS 180 PIKYNKDGSV 190 AIASKPREVR 200 EFNGQHFILE 210 EAITGDFALV 220 KAWKADRAGN 230 VIFRKSARNF 240 NLPMCKAAET 250 TVVEVEEIVD 260 IGAFAPEDIH 270 IPQIYVHRLI 280 KGEKYEKRIE 290 RLSIRKEGDG 300 EAKSAKPGDD 310 VRERIIKRAA 320 LEFEDGMYAN 330 LGIGIPLLAS 340 NFISPNITVH 350 LQSENGVLGL 360 GPYPRQHEAD 370 ADLINAGKET 380 VTILPGASFF 390 SSDESFAMIR 400 GGHVDLTMLG 410 AMQVSKYGDL 420 ANWMIPGKMV 430 KGMGGAMDLV 440 SSAKTKVVVT 450 MEHSAKGNAH 460 KIMEKCTLPL 470 TGKQCVNRII 480 TEKAVFDVDK 490 KKGLTLIELW 500 EGLTVDDVQK 510 STGCDFAVSP 520 KLMPMQQIAN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0008260 succinyl-CoA:3-oxo-acid CoA-transferase activity
Biological Process GO:0060612 adipose tissue development
Biological Process GO:0007507 heart development
Biological Process GO:0046952 ketone body catabolic process
Biological Process GO:1902224 ketone body metabolic process
Biological Process GO:0035774 positive regulation of insulin secretion involved in cellular response to glucose stimulus
Biological Process GO:0014823 response to activity
Biological Process GO:0045471 response to ethanol
Biological Process GO:0009725 response to hormone
Biological Process GO:0007584 response to nutrient
Biological Process GO:0042594 response to starvation
Biological Process GO:0009410 response to xenobiotic stimulus

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.