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Overview

Uniprot IDP56192
Protein NameMethionine--tRNA ligase, cytoplasmic
Gene NameMARS1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
866 LKAQKADKNEVAAEV

Function

Catalyzes the specific attachment of L-methionine to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285, PubMed:33909043). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971). In addition, can prevent the misincorporation of homocysteine into tRNA and protein by catalyzing a tRNA-independent hydrolysis of the misactivated homocysteinyl-AMP intermediate. The homocysteinyl-AMP intermediate undergoes an intramolecular cyclization reaction in which the thiolate side chain of homocysteine displaces the AMP group, forming homocysteine-thiolactone and AMP (By similarity)

Protein Sequence

10 MRLFVSDGVP 20 GCLPVLAAAG 30 RARGRAEVLI 40 STVGPEDCVV 50 PFLTRPKVPV 60 LQLDSGNYLF 70 STSAICRYFF 80 LLSGWEQDDL 90 TNQWLEWEAT 100 ELQPALSAAL 110 YYLVVQGKKG 120 EDVLGSVRRA 130 LTHIDHSLSR 140 QNCPFLAGET 150 ESLADIVLWG 160 ALYPLLQDPA 170 YLPEELSALH 180 SWFQTLSTQE 190 PCQRAAETVL 200 KQQGVLALRP 210 YLQKQPQPSP 220 AEGRAVTNEP 230 EEEELATLSE 240 EEIAMAVTAW 250 EKGLESLPPL 260 RPQQNPVLPV 270 AGERNVLITS 280 ALPYVNNVPH 290 LGNIIGCVLS 300 ADVFARYSRL 310 RQWNTLYLCG 320 TDEYGTATET 330 KALEEGLTPQ 340 EICDKYHIIH 350 ADIYRWFNIS 360 FDIFGRTTTP 370 QQTKITQDIF 380 QQLLKRGFVL 390 QDTVEQLRCE 400 HCARFLADRF 410 VEGVCPFCGY 420 EEARGDQCDK 430 CGKLINAVEL 440 KKPQCKVCRS 450 CPVVQSSQHL 460 FLDLPKLEKR 470 LEEWLGRTLP 480 GSDWTPNAQF 490 ITRSWLRDGL 500 KPRCITRDLK 510 WGTPVPLEGF 520 EDKVFYVWFD 530 ATIGYLSITA 540 NYTDQWERWW 550 KNPEQVDLYQ 560 FMAKDNVPFH 570 SLVFPCSALG 580 AEDNYTLVSH 590 LIATEYLNYE 600 DGKFSKSRGV 610 GVFGDMAQDT 620 GIPADIWRFY 630 LLYIRPEGQD 640 SAFSWTDLLL 650 KNNSELLNNL 660 GNFINRAGMF 670 VSKFFGGYVP 680 EMVLTPDDQR 690 LLAHVTLELQ 700 HYHQLLEKVR 710 IRDALRSILT 720 ISRHGNQYIQ 730 VNEPWKRIKG 740 SEADRQRAGT 750 VTGLAVNIAA 760 LLSVMLQPYM 770 PTVSATIQAQ 780 LQLPPPACSI 790 LLTNFLCTLP 800 AGHQIGTVSP 810 LFQKLENDQI 820 ESLRQRFGGG 830 QAKTSPKPAV 840 VETVTTAKPQ 850 QIQALMDEVT 860 KQGNIVRELK 870 AQKADKNEVA 880 AEVAKLLDLK 890 KQLAVAEGKP 900 PEAPKGKKKK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0017101 aminoacyl-tRNA synthetase multienzyme complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0016020 membrane
Cellular Component GO:0005730 nucleolus
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0004825 methionine-tRNA ligase activity
Molecular Function GO:0000049 tRNA binding
Biological Process GO:0071364 cellular response to epidermal growth factor stimulus
Biological Process GO:0036120 cellular response to platelet-derived growth factor stimulus
Biological Process GO:0050667 homocysteine metabolic process
Biological Process GO:0006431 methionyl-tRNA aminoacylation
Biological Process GO:1901838 positive regulation of transcription of nucleolar large rRNA by RNA polymerase I
Biological Process GO:0009303 rRNA transcription
Biological Process GO:0006418 tRNA aminoacylation for protein translation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.