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Overview

Uniprot IDP56382
Protein NameATP synthase F(1) complex subunit epsilon, mitochondrial
Gene NameAtp5f1e
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
21 RFSQICAKAVRDALK
32 DALKTEFKANAEKTS
37 EFKANAEKTSGSSIK
44 KTSGSSIKIVKVSKK

Function

Subunit epsilon, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (By similarity). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). May be essential for the assembly of F(1) and may play an important role in the incorporation of the hydrophobic subunit c into the F(1)-c oligomer rotor of the mitochondrial ATP synthase complex (By similarity)

Protein Sequence

10 MVAYWRQAGL 20 SYIRFSQICA 30 KAVRDALKTE 40 FKANAEKTSG 50 SSIKIVKVSK KE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0045259 proton-transporting ATP synthase complex
Molecular Function GO:0008553 P-type proton-exporting transporter activity
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0046933 proton-transporting ATP synthase activity, rotational mechanism
Biological Process GO:0015986 proton motive force-driven ATP synthesis
Biological Process GO:0042776 proton motive force-driven mitochondrial ATP synthesis

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.