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Overview

Uniprot ID	P56391
Protein Name	Cytochrome c oxidase subunit 6B1
Gene Name	Cox6b1
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
13	KTKIKNYKTAPFDSR
47	CEKAMTAKGGDVSVC

Function

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation (PubMed:34616041, PubMed:38575788). The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase (PubMed:34616041, PubMed:38575788). Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water (PubMed:34616041, PubMed:38575788). Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)) (PubMed:34616041, PubMed:38575788). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix (PubMed:34616041, PubMed:38575788)

Protein Sequence

10 MAEDIKTKIK 20 NYKTAPFDSR 30 FPNQNQTKNC 40 WQNYLDFHRC 50 EKAMTAKGGD 60 VSVCEWYRRV 70 YKSLCPVSWV 80 SAWDDRIAEG TFPGKI

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0031966	mitochondrial membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0045277	respiratory chain complex IV
Cellular Component	GO:0097228	sperm principal piece
Biological Process	GO:0006119	oxidative phosphorylation

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.