Search Results

Overview

Uniprot ID	P57772
Protein Name	Selenocysteine-specific elongation factor
Gene Name	EEFSEC
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
38	ASTAAFDKQPQSRER
397	DNDEADKKAGQATEG
549	KILTPALKKRARAGR

Function

Translation factor required for the incorporation of the rare amino acid selenocysteine encoded by UGA codons (PubMed:27708257, PubMed:35709277). Replaces the eRF1-eRF3-GTP ternary complex for the insertion of selenocysteine directed by the UGA codon (PubMed:27708257, PubMed:35709277). Insertion of selenocysteine at UGA codons is mediated by SECISBP2 and EEFSEC: SECISBP2 (1) specifically binds the SECIS sequence once the 80S ribosome encounters an in-frame UGA codon and (2) contacts the RPS27A/eS31 of the 40S ribosome before ribosome stalling (PubMed:35709277). (3) GTP-bound EEFSEC then delivers selenocysteinyl-tRNA(Sec) to the 80S ribosome and adopts a preaccommodated state conformation (PubMed:35709277). (4) After GTP hydrolysis, EEFSEC dissociates from the assembly, selenocysteinyl-tRNA(Sec) accommodates, and peptide bond synthesis and selenoprotein elongation occur (PubMed:35709277)

Protein Sequence

10 MAGRRVNVNV 20 GVLGHIDSGK 30 TALARALSTT 40 ASTAAFDKQP 50 QSRERGITLD 60 LGFSCFSVPL 70 PARLRSSLPE 80 FQAAPEAEPE 90 PGEPLLQVTL 100 VDCPGHASLI 110 RTIIGGAQII 120 DLMMLVIDVT 130 KGMQTQSAEC 140 LVIGQIACQK 150 LVVVLNKIDL 160 LPEGKRQAAI 170 DKMTKKMQKT 180 LENTKFRGAP 190 IIPVAAKPGG 200 PEAPETEAPQ 210 GIPELIELLT 220 SQISIPTRDP 230 SGPFLMSVDH 240 CFSIKGQGTV 250 MTGTILSGSI 260 SLGDSVEIPA 270 LKVVKKVKSM 280 QMFHMPITSA 290 MQGDRLGICV 300 TQFDPKLLER 310 GLVCAPESLH 320 TVHAALISVE 330 KIPYFRGPLQ 340 TKAKFHITVG 350 HETVMGRLMF 360 FSPAPDNFDQ 370 EPILDSFNFS 380 QEYLFQEQYL 390 SKDLTPAVTD 400 NDEADKKAGQ 410 ATEGHCPRQQ 420 WALVEFEKPV 430 TCPRLCLVIG 440 SRLDADIHTN 450 TCRLAFHGIL 460 LHGLEDRNYA 470 DSFLPRLKVY 480 KLKHKHGLVE 490 RAMDDYSVIG 500 RSLFKKETNI 510 QLFVGLKVHL 520 STGELGIIDS 530 AFGQSGKFKI 540 HIPGGLSPES 550 KKILTPALKK 560 RARAGRGEAT 570 RQEESAERSE 580 PSQHVVLSLT 590 FKRYVFDTHK RMVQSP

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:1990904	ribonucleoprotein complex
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0003924	GTPase activity
Molecular Function	GO:0043021	ribonucleoprotein complex binding
Molecular Function	GO:0035368	selenocysteine insertion sequence binding
Molecular Function	GO:0003746	translation elongation factor activity
Molecular Function	GO:0000049	tRNA binding
Biological Process	GO:0001514	selenocysteine incorporation

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.