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Overview

Uniprot IDP60710
Protein NameActin, cytoplasmic 1
Gene NameActb
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
113 TEAPLNPKANREKMT
328 APSTMKIKIIAPPER
50 VMVGMGQKDSYVGDE
61 VGDEAQSKRGILTLK

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (By similarity). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (By similarity). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:23558171, PubMed:25759381). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (By similarity). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity)

Protein Sequence

10 MDDDIAALVV 20 DNGSGMCKAG 30 FAGDDAPRAV 40 FPSIVGRPRH 50 QGVMVGMGQK 60 DSYVGDEAQS 70 KRGILTLKYP 80 IEHGIVTNWD 90 DMEKIWHHTF 100 YNELRVAPEE 110 HPVLLTEAPL 120 NPKANREKMT 130 QIMFETFNTP 140 AMYVAIQAVL 150 SLYASGRTTG 160 IVMDSGDGVT 170 HTVPIYEGYA 180 LPHAILRLDL 190 AGRDLTDYLM 200 KILTERGYSF 210 TTTAEREIVR 220 DIKEKLCYVA 230 LDFEQEMATA 240 ASSSSLEKSY 250 ELPDGQVITI 260 GNERFRCPEA 270 LFQPSFLGME 280 SCGIHETTFN 290 SIMKCDVDIR 300 KDLYANTVLS 310 GGTTMYPGIA 320 DRMQKEITAL 330 APSTMKIKII 340 APPERKYSVW 350 IGGSILASLS 360 TFQQMWISKQ 370 EYDESGPSIV HRKCF

Gene Ontology

Classification GO ID Description
Cellular Component GO:0015629 actin cytoskeleton
Cellular Component GO:0005884 actin filament
Cellular Component GO:0005912 adherens junction
Cellular Component GO:0043296 apical junction complex
Cellular Component GO:0030424 axon
Cellular Component GO:0005903 brush border
Cellular Component GO:0044305 calyx of Held
Cellular Component GO:0005911 cell-cell junction
Cellular Component GO:0000785 chromatin
Cellular Component GO:0030863 cortical cytoskeleton
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0036464 cytoplasmic ribonucleoprotein granule
Cellular Component GO:0005856 cytoskeleton
Cellular Component GO:0005829 cytosol
Cellular Component GO:0097433 dense body
Cellular Component GO:0005925 focal adhesion
Cellular Component GO:0098978 glutamatergic synapse
Cellular Component GO:0000776 kinetochore
Cellular Component GO:0030027 lamellipodium
Cellular Component GO:0016020 membrane
Cellular Component GO:0045121 membrane raft
Cellular Component GO:0043209 myelin sheath
Cellular Component GO:0035267 NuA4 histone acetyltransferase complex
Cellular Component GO:0016363 nuclear matrix
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0000786 nucleosome
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0002102 podosome
Cellular Component GO:0098871 postsynaptic actin cytoskeleton
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:1990904 ribonucleoprotein complex
Cellular Component GO:0098685 Schaffer collateral - CA1 synapse
Cellular Component GO:0001725 stress fiber
Cellular Component GO:0045202 synapse
Cellular Component GO:0070160 tight junction
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0019894 kinesin binding
Molecular Function GO:0050998 nitric-oxide synthase binding
Molecular Function GO:0030235 nitric-oxide synthase regulator activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0098973 structural constituent of postsynaptic actin cytoskeleton
Molecular Function GO:0030957 Tat protein binding
Molecular Function GO:0141108 transporter regulator activity
Biological Process GO:0034333 adherens junction assembly
Biological Process GO:0045176 apical protein localization
Biological Process GO:0007409 axonogenesis
Biological Process GO:0048870 cell motility
Biological Process GO:0072749 cellular response to cytochalasin B
Biological Process GO:0071257 cellular response to electrical stimulus
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0007010 cytoskeleton organization
Biological Process GO:0007163 establishment or maintenance of cell polarity
Biological Process GO:0001738 morphogenesis of a polarized epithelium
Biological Process GO:0045596 negative regulation of cell differentiation
Biological Process GO:0045597 positive regulation of cell differentiation
Biological Process GO:0008284 positive regulation of cell population proliferation
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:2000781 positive regulation of double-strand break repair
Biological Process GO:1905168 positive regulation of double-strand break repair via homologous recombination
Biological Process GO:0045663 positive regulation of myoblast differentiation
Biological Process GO:1902459 positive regulation of stem cell population maintenance
Biological Process GO:0045582 positive regulation of T cell differentiation
Biological Process GO:0071896 protein localization to adherens junction
Biological Process GO:0042981 regulation of apoptotic process
Biological Process GO:0051726 regulation of cell cycle
Biological Process GO:2000779 regulation of double-strand break repair
Biological Process GO:0070316 regulation of G0 to G1 transition
Biological Process GO:2000045 regulation of G1/S transition of mitotic cell cycle
Biological Process GO:0030071 regulation of mitotic metaphase/anaphase transition
Biological Process GO:0051621 regulation of norepinephrine uptake
Biological Process GO:2000819 regulation of nucleotide-excision repair
Biological Process GO:1903076 regulation of protein localization to plasma membrane
Biological Process GO:1900242 regulation of synaptic vesicle endocytosis
Biological Process GO:0006357 regulation of transcription by RNA polymerase II
Biological Process GO:0150111 regulation of transepithelial transport

Reference

[1] Sung E, Sim H, Cho YC, Lee W, Bae JS et al.. Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells.. J Proteome Res 22(12):3683-3691. 2023 Dec 1. PMID: 37897433.

[2] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[3] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.