Search Results
Overview
| Uniprot ID | P61088 |
|---|---|
| Protein Name | Ubiquitin-conjugating enzyme E2 N |
| Gene Name | UBE2N |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 10 | GLPRRIIKETQRLLA |
| 74 | PKVRFMTKIYHPNVD |
| 92 | RICLDILKDKWSPAL |
| 94 | CLDILKDKWSPALQI |
Function
The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175, PubMed:31006531). UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0070062 | extracellular exosome |
| Cellular Component | GO:0001650 | fibrillar center |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0032991 | protein-containing complex |
| Cellular Component | GO:0031372 | UBC13-MMS2 complex |
| Cellular Component | GO:0031371 | ubiquitin conjugating enzyme complex |
| Cellular Component | GO:0000151 | ubiquitin ligase complex |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0043130 | ubiquitin binding |
| Molecular Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Molecular Function | GO:0031625 | ubiquitin protein ligase binding |
| Molecular Function | GO:0097027 | ubiquitin-protein transferase activator activity |
| Molecular Function | GO:0004842 | ubiquitin-protein transferase activity |
| Biological Process | GO:0140374 | antiviral innate immune response |
| Biological Process | GO:0006301 | DNA damage tolerance |
| Biological Process | GO:0000729 | DNA double-strand break processing |
| Biological Process | GO:0000724 | double-strand break repair via homologous recombination |
| Biological Process | GO:1904262 | negative regulation of TORC1 signaling |
| Biological Process | GO:0043123 | positive regulation of canonical NF-kappaB signal transduction |
| Biological Process | GO:0045739 | positive regulation of DNA repair |
| Biological Process | GO:2000781 | positive regulation of double-strand break repair |
| Biological Process | GO:1902533 | positive regulation of intracellular signal transduction |
| Biological Process | GO:1902523 | positive regulation of protein K63-linked ubiquitination |
| Biological Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Biological Process | GO:0070534 | protein K63-linked ubiquitination |
| Biological Process | GO:0006513 | protein monoubiquitination |
| Biological Process | GO:0000209 | protein polyubiquitination |
| Biological Process | GO:0016567 | protein ubiquitination |
| Biological Process | GO:0006282 | regulation of DNA repair |
| Biological Process | GO:0050852 | T cell receptor signaling pathway |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.
[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.
[4] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.
[5] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.
[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.